HitPredict

Interactor 1: VPS2B (Q0WTY4)
Vacuolar protein sorting-associated protein 2 homolog 2 (AtVPS2-2) (Charged multivesicular body protein 2 homolog 2) (ESCRT-III complex subunit VPS2 homolog 2)
Gene: VPS2.2

Interactor 2: VP322 (Q9SZE4)
Vacuolar protein sorting-associated protein 32 homolog 2 (AtVPS32-2) (Charged multivesicular body protein 4 homolog 2) (ESCRT-III complex subunit VPS32 homolog 2)
Gene: VPS32.2

Species: Arabidopsis thaliana

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.69	1.00	0.836

Property	Details
Experiments	22010978, 21442383
Identification Methods	affinity chromatography technology, two hybrid, protein complementation assay, anti tag coimmunoprecipitation, bimolecular fluorescence complementation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Snf7~Snf7
Common GO terms	GO:0000815 - ESCRT III complex GO:0005771 - multivesicular body GO:0015031 - protein transport
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 222 At			1 219
+	Ce
		1090380 Q9U2F6_CAEEL (3-221): 7.98e-54: 174: 31.33		Q18886_CAEEL (1-218): 5.1e-44: 148: 35.00
+	At
		1276050 VPS2C_ARATH (1-220): 3.63e-54: 174: 68.64		VP322_ARATH (1-219): 2.31e-43: 146: 100.00
+	At
		1013158 VPS2B_ARATH (1-222): 2.69e-53: 172: 100.00		VP601_ARATH (2-211): 2.8e-42: 144: 28.13
+	Dm
		1249819 Q9VN02_DROME (1-220): 1.53e-50: 165: 24.32		CHM4_DROME (1-218): 1.33e-40: 139: 27.93
+	At
		287069 VPS2C_ARATH (1-220): 3.63e-54: 174: 68.64		VP321_ARATH (1-219): 6.2e-37: 130: 79.91
+	At
		287066 VPS2B_ARATH (1-222): 2.69e-53: 172: 100.00		VP321_ARATH (1-219): 6.2e-37: 130: 79.91
+	At
		1276045 VPS2C_ARATH (1-220): 3.63e-54: 174: 68.64		VP202_ARATH (2-216): 6.25e-33: 119: 24.19
+	Hs
		1330574 CHMP3_HUMAN (1-220): 1.47e-48: 160: 22.97		CHM4B_HUMAN (13-218): 4.66e-38: 133: 37.86
+	At
		1209623 VPS2C_ARATH (1-220): 3.63e-54: 174: 68.64		VP201_ARATH (2-216): 5.8e-32: 117: 23.85
+	Dm
		1371192 Q9VN02_DROME (1-220): 1.53e-50: 165: 24.32		CHMP5_DROME (2-211): 1.21e-34: 124: 28.18
+	Hs
		1349478 CHMP3_HUMAN (1-220): 1.47e-48: 160: 22.97		CHMP5_HUMAN (2-193): 2.04e-35: 126: 30.20
+	Sc
		732845 DID4_YEAST (3-221): 2.54e-51: 167: 33.04		SNF7_YEAST (1-208): 2.43e-32: 119: 27.70
+	Hs
		1316088 CHMP3_HUMAN (1-220): 1.47e-48: 160: 22.97		CHM4A_HUMAN (2-217): 1e-33: 122: 35.32
+	Sc
		732860 DID4_YEAST (3-221): 2.54e-51: 167: 33.04		VPS60_YEAST (8-216): 7.64e-30: 112: 21.33
+	Dm
		1371191 Q9VN02_DROME (1-220): 1.53e-50: 165: 24.32		Q9VRJ5_DROME (1-216): 2.05e-30: 113: 14.29
+	Sc
		732861 DID4_YEAST (3-221): 2.54e-51: 167: 33.04		VPS20_YEAST (8-219): 5.68e-28: 107: 17.97
+	Hs
		1357921 CHMP3_HUMAN (1-220): 1.47e-48: 160: 22.97		CHM2B_HUMAN (2-213): 7.43e-29: 109: 16.04
+	At
		1013164 VPS2A_ARATH (2-222): 6.22e-58: 184: 40.99		VPS2B_ARATH (8-217): 9.42e-15: 72.1: 15.81
+	At
		1013141 VPS2B_ARATH (1-222): 2.69e-53: 172: 100.00		VPS2B_ARATH (8-217): 9.42e-15: 72.1: 15.81