HitPredict

Interactor 1: AP1B1 (Q10567)
AP-1 complex subunit beta-1 (Adaptor protein complex AP-1 subunit beta-1) (Adaptor-related protein complex 1 subunit beta-1) (Beta-1-adaptin) (Beta-adaptin 1) (Clathrin assembly protein complex 1 beta large chain) (Golgi adaptor HA1/AP1 adaptin beta subunit)
Gene: AP1B1

Interactor 2: EPN4 (Q14677)
Clathrin interactor 1 (Clathrin-interacting protein localized in the trans-Golgi region) (Clint) (Enthoprotin) (Epsin-4) (Epsin-related protein) (EpsinR)
Gene: CLINT1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.80	0.60	0.696

Property	Details
Experiments	28514442, 12429846, 33961781, 12538641
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005794 - Golgi apparatus GO:0005829 - cytosol GO:0030276 - clathrin binding GO:0043231 - intracellular membrane-bounded organelle
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 949 Hs			1 625
+	Hs
		925867 AP2B1_HUMAN (1-949): 0: 944: 81.45		EPN4_HUMAN (1-625): 2.12e-141: 426: 100.00
+	Hs
		1014105 AP1B1_HUMAN (1-949): 0: 978: 100.00		EPN1_HUMAN (8-529): 2.46e-67: 231: 23.55
+	Rn
		923862 AP2B1_RAT (1-949): 0: 944: 81.45		EPN2_RAT (8-556): 7.08e-74: 249: 19.17
+	Rn
		291389 AP2B1_RAT (1-949): 0: 944: 81.45		EPN1_RAT (8-529): 1.01e-68: 235: 24.63
+	Hs
		926065 AP2B1_HUMAN (1-949): 0: 944: 81.45		EPN1_HUMAN (8-529): 2.46e-67: 231: 23.55
+	Dm
		1096902 Q24253_DROME (1-949): 0: 851: 72.74		Q8IN05_DROME (2-473): 8.16e-95: 320: 34.92
+	Hs
		307524 AP2B1_HUMAN (1-949): 0: 944: 81.45		EPN2_HUMAN (8-595): 6.55e-52: 191: 20.40
+	Hs
		203223 AP1B1_HUMAN (1-949): 0: 978: 100.00		SYNJ1_HUMAN (120-617): 7.92e-35: 143: 10.29
+	Hs
		321545 AP2A1_HUMAN (12-943): 0: 677: 18.58		EPN4_HUMAN (1-625): 2.12e-141: 426: 100.00
+	Dm
		1096905 Q24253_DROME (1-949): 0: 851: 72.74		Q9VD13_DROME (2-551): 1.21e-72: 247: 31.88
+	Hs
		203219 AP2B1_HUMAN (1-949): 0: 944: 81.45		SYNJ1_HUMAN (120-617): 7.92e-35: 143: 10.29
+	Hs
		302148 AP2A2_HUMAN (12-945): 0: 625: 18.33		EPN4_HUMAN (1-625): 2.12e-141: 426: 100.00
+	Hs
		321680 AP2A1_HUMAN (12-943): 0: 677: 18.58		EPN1_HUMAN (8-529): 2.46e-67: 231: 23.55
+	Rn
		88757 D3ZUY8_RAT (12-943): 0: 670: 18.03		EPN1_RAT (8-529): 1.01e-68: 235: 24.63
+	Rn
		291387 AP2A2_RAT (12-945): 0: 635: 17.94		EPN1_RAT (8-529): 1.01e-68: 235: 24.63
+	Rn
		291395 Q66HM2_RAT (12-945): 0: 631: 17.92		EPN1_RAT (8-529): 1.01e-68: 235: 24.63
+	At
		1228257 AP2A1_ARATH (13-946): 0: 627: 16.87		EPN1_ARATH (7-421): 1.05e-66: 229: 21.81
+	Hs
		203194 AP2A1_HUMAN (12-943): 0: 677: 18.58		SYNJ1_HUMAN (120-617): 7.92e-35: 143: 10.29
+	At
		1147101 AP2A1_ARATH (13-946): 0: 627: 16.87		EPN2_ARATH (7-612): 4.91e-48: 182: 17.53
+	Rn
		1144064 Q66HM2_RAT (12-945): 0: 631: 17.92		SYNJ1_RAT (314-625): 4.54e-10: 65: 10.35