HitPredict

Interactor 1: SMAD4 (Q13485)
Mothers against decapentaplegic homolog 4 (MAD homolog 4) (Mothers against DPP homolog 4) (Deletion target in pancreatic carcinoma 4) (SMAD family member 4) (SMAD 4) (Smad4) (hSMAD4)
Gene: SMAD4

Interactor 2: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Species: Homo sapiens

Source: biogrid

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.38	0.60	0.479

Property	Details
Experiments	31932471
Identification Methods	affinity chromatography technology
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 552 Hs			1 903
+	Hs
		1035583 SMAD4_HUMAN (1-552): 0: 696: 100.00		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		950248 SMAD3_HUMAN (19-549): 1.96e-176: 506: 31.22		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Hs
		1078757 SMAD1_HUMAN (20-546): 0: 542: 34.91		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		1305881 SMAD5_HUMAN (20-546): 0: 537: 34.15		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		950278 SMAD3_HUMAN (19-549): 1.96e-176: 506: 31.22		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125369 SMAD3_HUMAN (19-549): 1.96e-176: 506: 31.22		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		950249 SMAD3_HUMAN (19-549): 1.96e-176: 506: 31.22		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		808135 SMAD1_HUMAN (20-546): 0: 542: 34.91		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		808240 SMAD5_HUMAN (20-546): 0: 537: 34.15		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		808087 SMAD3_HUMAN (19-549): 1.96e-176: 506: 31.22		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		1035600 SMAD4_HUMAN (1-552): 0: 696: 100.00		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		1035596 SMAD4_HUMAN (1-552): 0: 696: 100.00		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Hs
		1078761 SMAD1_HUMAN (20-546): 0: 542: 34.91		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Mm
		56826 SMAD1_MOUSE (20-546): 0: 544: 34.72		SMUF2_MOUSE (1-358): 2.99e-46: 179: 16.67
+	Hs
		1078760 SMAD1_HUMAN (20-546): 0: 542: 34.91		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Hs
		1305888 SMAD5_HUMAN (20-546): 0: 537: 34.15		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		1305886 SMAD5_HUMAN (20-546): 0: 537: 34.15		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Mm
		936618 SMAD1_MOUSE (20-546): 0: 544: 34.72		SMUF1_MOUSE (1-535): 3.62e-41: 164: 14.07
+	Xl
		945613 P79947_XENLA (20-546): 0: 544: 34.15		SMUF1_XENLA (261-535): 3.55e-12: 72.2: 15.54
+	Hs
		1305892 SMAD5_HUMAN (20-546): 0: 537: 34.15		HECW2_HUMAN (1-512): 1.38e-13: 77.2: 11.34