HitPredict

Interactor 1: CUL1 (Q13616)
Cullin-1 (CUL-1)
Gene: CUL1

Interactor 2: NEDD8 (Q15843)
NEDD8 (Neddylin) (Neural precursor cell expressed developmentally down-regulated protein 8) (NEDD-8) (Ubiquitin-like protein Nedd8)
Gene: NEDD8

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.96	1.00	0.980

Property	Details
Experiments	16503656, 21145461, 35713976, 22474075, 18723677, 18264111, 21765416, 10713156, 19713960, 17318178, 18274552, 21102408
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation, comigration in sds page, enzymatic study, tandem affinity purification, anti bait coimmunoprecipitation
Interaction Type	physical association, covalent binding, ubiquitination reaction, direct interaction, association

Property	Details
Interacting Pfam domains	Cullin~ubiquitin, Cullin_Nedd8~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 776 Hs			1 81
+	Hs
		923984 CUL4A_HUMAN (7-776): 0: 781: 30.41		RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14
+	Hs
		923985 CUL4B_HUMAN (7-776): 0: 773: 29.90		RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14
+	Hs
		924296 CUL4A_HUMAN (7-776): 0: 781: 30.41		RL40_HUMAN (1-76): 3.79e-33: 112: 57.90
+	Hs
		1043010 CUL4A_HUMAN (7-776): 0: 781: 30.41		NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00
+	Dm
		1097203 CUL1_DROME (4-776): 0: 770: 61.98		NEDD8_DROME (1-76): 2.21e-34: 113: 93.42
+	Hs
		475024 CUL4B_HUMAN (7-776): 0: 773: 29.90		UBB_HUMAN (1-76): 2.4e-30: 107: 57.90
+	Hs
		1043371 CUL4B_HUMAN (7-776): 0: 773: 29.90		NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00
+	At
		1352191 CUL3A_ARATH (7-776): 0: 781: 30.21		RUB1_ARATH (3-76): 7.75e-26: 94.5: 85.14
+	Hs
		923983 CUL3_HUMAN (9-776): 0: 748: 30.47		RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14
+	At
		1321116 CUL3B_ARATH (7-776): 0: 768: 29.69		RUB1_ARATH (3-76): 7.75e-26: 94.5: 85.14
+	Hs
		1041658 CUL3_HUMAN (9-776): 0: 748: 30.47		NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00
+	Dm
		1213846 Q8IP45_DROME (10-776): 0: 729: 30.03		NEDD8_DROME (1-76): 2.21e-34: 113: 93.42
+	Sp
		142907 CUL1_SCHPO (1-776): 0: 733: 40.92		UBL1_SCHPO (1-76): 9.64e-30: 102: 81.58
+	Hs
		1041337 CUL2_HUMAN (13-776): 0: 720: 35.98		NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00
+	At
		1277343 CUL1_ARATH (10-776): 0: 722: 30.29		RUB1_ARATH (3-76): 7.75e-26: 94.5: 85.14
+	At
		1247030 CUL1_ARATH (10-776): 0: 722: 30.29		RUB2_ARATH (3-76): 1.03e-24: 91.8: 85.14
+	Ce
		1088883 CUL3_CAEEL (10-776): 0: 670: 27.06		NEDD8_CAEEL (1-77): 7.56e-34: 112: 87.01
+	Sp
		145590 CUL3_SCHPO (5-776): 0: 635: 26.43		UBL1_SCHPO (1-76): 9.64e-30: 102: 81.58
+	Hs
		1079566 CUL5_HUMAN (2-776): 0: 622: 28.85		NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00
+	Sc
		977843 CDC53_YEAST (13-776): 0: 628: 31.36		RUB1_YEAST (1-76): 1.45e-21: 81.3: 59.21