HitPredict

Interactor 1: CUL3 (Q13618)
Cullin-3 (CUL-3)
Gene: CUL3

Interactor 2: KLH42 (Q9P2K6)
Kelch-like protein 42 (Cullin-3-binding protein 9) (Ctb9) (Kelch domain-containing protein 5)
Gene: KLHL42

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.980

Property	Details
Experiments	28514442, 33130828, 33961781, 34878901, 35563538, 19261606, 21145461, 19256485, 19295130, 25011449, 34591642, 30190310, 37207277
Identification Methods	affinity chromatography technology, two hybrid, pull down, tandem affinity purification, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	BACK~Cullin
Common GO terms	GO:0000209 - protein polyubiquitination GO:0005829 - cytosol GO:0031463 - Cul3-RING ubiquitin ligase complex GO:0043161 - proteasome-mediated ubiquitin-dependent protein catabolic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 768 Hs			1 505
+	Hs
		1041715 CUL3_HUMAN (1-768): 0: 951: 100.00		KLH22_HUMAN (6-501): 8.93e-110: 341: 19.21
+	Hs
		1042027 CUL3_HUMAN (1-768): 0: 951: 100.00		KLH36_HUMAN (6-504): 5.8e-108: 335: 20.84
+	Hs
		1041547 CUL3_HUMAN (1-768): 0: 951: 100.00		KEAP1_HUMAN (4-504): 6.64e-101: 317: 15.75
+	Hs
		299515 CUL3_HUMAN (1-768): 0: 951: 100.00		KLH18_HUMAN (2-498): 3.63e-100: 314: 17.40
+	Hs
		1041852 CUL3_HUMAN (1-768): 0: 951: 100.00		KLH17_HUMAN (2-499): 2.6e-92: 296: 16.02
+	Rn
		78189 CUL3_RAT (1-768): 0: 951: 99.61		KLH17_RAT (2-499): 1.13e-92: 296: 16.37
+	Hs
		1041718 CUL3_HUMAN (1-768): 0: 951: 100.00		KLH26_HUMAN (4-504): 1.01e-88: 285: 19.30
+	Hs
		1041853 CUL3_HUMAN (1-768): 0: 951: 100.00		KLH24_HUMAN (3-503): 6.17e-88: 283: 16.70
+	Hs
		1041712 CUL3_HUMAN (1-768): 0: 951: 100.00		KLH12_HUMAN (56-450): 1.45e-54: 194: 13.03
+	Hs
		1042674 CUL3_HUMAN (1-768): 0: 951: 100.00		KLH28_HUMAN (60-447): 9.61e-52: 186: 16.37
+	Hs
		1042057 CUL3_HUMAN (1-768): 0: 951: 100.00		KLH23_HUMAN (171-392): 6.43e-35: 139: 23.89
+	Hs
		1041807 CUL3_HUMAN (1-768): 0: 951: 100.00		KLH10_HUMAN (329-498): 3.32e-26: 114: 14.04
+	Hs
		307170 CUL3_HUMAN (1-768): 0: 951: 100.00		KLHL2_HUMAN (172-346): 7.92e-23: 103: 23.89
+	Hs
		1042083 CUL3_HUMAN (1-768): 0: 951: 100.00		KBTB8_HUMAN (142-479): 1.17e-20: 97.4: 26.36
+	Hs
		1042324 CUL3_HUMAN (1-768): 0: 951: 100.00		KLHL5_HUMAN (372-500): 5.25e-18: 89.2: 18.61
+	Hs
		1042624 CUL3_HUMAN (1-768): 0: 951: 100.00		KLHL1_HUMAN (359-500): 1.3e-17: 88: 17.57
+	Hs
		1042489 CUL3_HUMAN (1-768): 0: 951: 100.00		KLHL4_HUMAN (377-500): 3.09e-16: 83.8: 19.36
+	Hs
		296838 CUL3_HUMAN (1-768): 0: 951: 100.00		KBTBB_HUMAN (351-487): 4.5e-11: 67.1: 13.67
+	Hs
		87524 CUL3_HUMAN (1-768): 0: 951: 100.00		KBTBD_HUMAN (362-495): 4.15e-11: 67.1: 13.67
+	Hs
		1042167 CUL3_HUMAN (1-768): 0: 951: 100.00		KLHL6_HUMAN (238-377): 9.69e-09: 59.7: 26.06