HitPredict

Interactor 1: CUL4B (Q13620)
Cullin-4B (CUL-4B)
Gene: CUL4B

Interactor 2: AMRA1 (Q9C0C7)
Activating molecule in BECN1-regulated autophagy protein 1 (DDB1- and CUL4-associated factor 3)
Gene: AMBRA1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.92	0.60	0.746

Property	Details
Experiments	25499913, 30166453, 31973889, 33854239, 16949367, 31155351, 21145461
Identification Methods	enzymatic study, affinity chromatography technology, pull down, tandem affinity purification
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000209 - protein polyubiquitination GO:0005634 - nucleus GO:0005829 - cytosol GO:0031625 - ubiquitin protein ligase binding GO:0080008 - Cul4-RING E3 ubiquitin ligase complex
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 913 Hs			1 1298
+	Hs
		1043205 CUL4A_HUMAN (161-913): 0: 955: 81.83		AMRA1_HUMAN (1-1298): 0: 1603: 100.00
+	Hs
		903209 CUL4B_HUMAN (1-913): 0: 1184: 100.00		WDR5_HUMAN (3-297): 2.47e-87: 289: 18.39
+	Hs
		1043441 CUL4B_HUMAN (1-913): 0: 1184: 100.00		WDR5B_HUMAN (5-294): 1.05e-85: 284: 17.38
+	Hs
		1043517 CUL4B_HUMAN (1-913): 0: 1184: 100.00		DCAF5_HUMAN (13-774): 2.83e-71: 260: 14.74
+	Hs
		1043509 CUL4B_HUMAN (1-913): 0: 1184: 100.00		SNR40_HUMAN (45-329): 6.16e-55: 197: 11.75
+	Hs
		1043499 CUL4B_HUMAN (1-913): 0: 1184: 100.00		FBXW7_HUMAN (164-329): 5.74e-18: 91.8: 9.58
+	Hs
		1043469 CUL4B_HUMAN (1-913): 0: 1184: 100.00		POC1B_HUMAN (1-83): 1.05e-16: 86.8: 16.28
+	Hs
		152876 CUL4B_HUMAN (1-913): 0: 1184: 100.00		APAF_HUMAN (33-203): 4.64e-16: 86.3: 19.23
+	Hs
		245601 CUL4B_HUMAN (1-913): 0: 1184: 100.00		TBL1X_HUMAN (28-159): 4.53e-15: 82.1: 20.14
+	Hs
		1043562 CUL4B_HUMAN (1-913): 0: 1184: 100.00		TBL1R_HUMAN (28-201): 1.6e-14: 80.1: 17.98
+	Hs
		903208 CUL4A_HUMAN (161-913): 0: 955: 81.83		WDR5_HUMAN (3-297): 2.47e-87: 289: 18.39
+	Hs
		1043085 CUL4A_HUMAN (161-913): 0: 955: 81.83		WDR5B_HUMAN (5-294): 1.05e-85: 284: 17.38
+	Hs
		1043162 CUL4A_HUMAN (161-913): 0: 955: 81.83		DCAF5_HUMAN (13-774): 2.83e-71: 260: 14.74
+	Hs
		1043157 CUL4A_HUMAN (161-913): 0: 955: 81.83		SNR40_HUMAN (45-329): 6.16e-55: 197: 11.75
+	Hs
		792982 CUL4A_HUMAN (161-913): 0: 955: 81.83		LIS1_HUMAN (123-346): 3.66e-35: 141: 11.45
+	At
		95048 CUL4_ARATH (147-913): 0: 919: 55.73		KTN82_ARATH (126-797): 3.69e-37: 154: 11.79
+	Hs
		1043114 CUL4A_HUMAN (161-913): 0: 955: 81.83		POC1B_HUMAN (1-83): 1.05e-16: 86.8: 16.28
+	Hs
		1043340 CUL4A_HUMAN (161-913): 0: 955: 81.83		WSB1_HUMAN (57-256): 4.52e-15: 81.3: 13.51
+	Hs
		1043262 CUL4A_HUMAN (161-913): 0: 955: 81.83		WSB2_HUMAN (41-201): 1.66e-13: 75.9: 23.67
+	Hs
		152875 CUL3_HUMAN (186-913): 0: 853: 37.19		APAF_HUMAN (33-203): 4.64e-16: 86.3: 19.23