HitPredict

Interactor 1: SMAD2 (Q15796)
Mothers against decapentaplegic homolog 2 (MAD homolog 2) (Mothers against DPP homolog 2) (JV18-1) (Mad-related protein 2) (hMAD-2) (SMAD family member 2) (SMAD 2) (Smad2) (hSMAD2)
Gene: SMAD2

Interactor 2: PIAS4 (Q8N2W9)
E3 SUMO-protein ligase PIAS4 (EC 2.3.2.27) (PIASy) (Protein inhibitor of activated STAT protein 4) (Protein inhibitor of activated STAT protein gamma) (PIAS-gamma)
Gene: PIAS4

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.64	0.60	0.620

Property	Details
Experiments	12904571, 12815042
Identification Methods	two hybrid, affinity chromatography technology
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0031625 - ubiquitin protein ligase binding GO:0045892 - negative regulation of DNA-templated transcription
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 467 Hs			1 510
+	Hs
		950220 SMAD3_HUMAN (1-467): 0: 617: 83.94		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Hs
		1078729 SMAD1_HUMAN (1-467): 0: 565: 55.53		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Hs
		1078712 SMAD2_HUMAN (1-467): 0: 675: 100.00		PIAS3_HUMAN (3-509): 0: 540: 46.59
+	Hs
		950349 SMAD3_HUMAN (1-467): 0: 617: 83.94		PIAS3_HUMAN (3-509): 0: 540: 46.59
+	Hs
		278264 SMAD3_HUMAN (1-467): 0: 617: 83.94		PIAS2_HUMAN (3-504): 9.32e-180: 520: 44.74
+	Hs
		1035500 SMAD4_HUMAN (11-456): 4.53e-151: 443: 31.58		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Hs
		278098 SMAD1_HUMAN (1-467): 0: 565: 55.53		PIAS1_HUMAN (3-508): 2.66e-180: 522: 46.42
+	Hs
		167374 SMAD7_HUMAN (13-443): 2.55e-109: 331: 25.23		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Hs
		206968 SMAD6_HUMAN (38-445): 3.64e-108: 331: 26.57		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Hs
		1035653 SMAD4_HUMAN (11-456): 4.53e-151: 443: 31.58		PIAS3_HUMAN (3-509): 0: 540: 46.59
+	Hs
		278075 SMAD4_HUMAN (11-456): 4.53e-151: 443: 31.58		PIAS1_HUMAN (3-508): 2.66e-180: 522: 46.42
+	Hs
		278278 SMAD4_HUMAN (11-456): 4.53e-151: 443: 31.58		PIAS2_HUMAN (3-504): 9.32e-180: 520: 44.74
+	Hs
		206981 SMAD6_HUMAN (38-445): 3.64e-108: 331: 26.57		PIAS3_HUMAN (3-509): 0: 540: 46.59
+	Hs
		950322 SMAD3_HUMAN (1-467): 0: 617: 83.94		ZMIZ1_HUMAN (73-419): 2.68e-71: 247: 23.42
+	Hs
		167316 SMAD7_HUMAN (13-443): 2.55e-109: 331: 25.23		PIAS1_HUMAN (3-508): 2.66e-180: 522: 46.42
+	Hs
		1035630 SMAD4_HUMAN (11-456): 4.53e-151: 443: 31.58		ZMIZ1_HUMAN (73-419): 2.68e-71: 247: 23.42