HitPredict

Interactor 1: SMAD2 (Q15796)
Mothers against decapentaplegic homolog 2 (MAD homolog 2) (Mothers against DPP homolog 2) (JV18-1) (Mad-related protein 2) (hMAD-2) (SMAD family member 2) (SMAD 2) (Smad2) (hSMAD2)
Gene: SMAD2

Interactor 2: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.52	0.60	0.561

Property	Details
Experiments	15350225
Identification Methods	enzymatic study, affinity chromatography technology
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0032991 - protein-containing complex
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 467 Hs			1 903
+	Mm
		1143948 SMAD2_MOUSE (1-467): 0: 675: 99.57		ITCH_MOUSE (1-903): 0: 885: 91.16
+	Hs
		950248 SMAD3_HUMAN (1-467): 0: 617: 83.94		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Hs
		1078654 SMAD2_HUMAN (1-467): 0: 675: 100.00		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125487 SMAD2_HUMAN (1-467): 0: 675: 100.00		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		950278 SMAD3_HUMAN (1-467): 0: 617: 83.94		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		1078629 SMAD2_HUMAN (1-467): 0: 675: 100.00		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		125369 SMAD3_HUMAN (1-467): 0: 617: 83.94		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		1305881 SMAD5_HUMAN (1-467): 0: 570: 55.45		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		950249 SMAD3_HUMAN (1-467): 0: 617: 83.94		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Dm
		785516 MAD_DROME (1-467): 0: 586: 55.90		SUDX_DROME (19-903): 0: 788: 48.29
+	Hs
		808134 SMAD2_HUMAN (1-467): 0: 675: 100.00		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		808087 SMAD3_HUMAN (1-467): 0: 617: 83.94		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		808240 SMAD5_HUMAN (1-467): 0: 570: 55.45		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		1078671 SMAD2_HUMAN (1-467): 0: 675: 100.00		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		1078667 SMAD2_HUMAN (1-467): 0: 675: 100.00		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Hs
		950291 SMAD3_HUMAN (1-467): 0: 617: 83.94		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		950287 SMAD3_HUMAN (1-467): 0: 617: 83.94		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Hs
		1305886 SMAD5_HUMAN (1-467): 0: 570: 55.45		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Dm
		785477 MAD_DROME (1-467): 0: 586: 55.90		SMUF1_DROME (16-518): 1.15e-39: 161: 16.19
+	Xl
		945613 P79947_XENLA (1-467): 0: 571: 55.64		SMUF1_XENLA (261-535): 3.55e-12: 72.2: 15.54