HitPredict

Interactor 1: SMAD2 (Q15796)
Mothers against decapentaplegic homolog 2 (MAD homolog 2) (Mothers against DPP homolog 2) (JV18-1) (Mad-related protein 2) (hMAD-2) (SMAD family member 2) (SMAD 2) (Smad2) (hSMAD2)
Gene: SMAD2

Interactor 2: TRI33 (Q9UPN9)
E3 ubiquitin-protein ligase TRIM33 (EC 2.3.2.27) (Ectodermin homolog) (RET-fused gene 7 protein) (Protein Rfg7) (RING-type E3 ubiquitin transferase TRIM33) (Transcription intermediary factor 1-gamma) (TIF1-gamma) (Tripartite motif-containing protein 33)
Gene: TRIM33

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.87	0.60	0.724

Property	Details
Experiments	16751102, 22196728, 18003620, 23788427, 19135894, 20603019
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0017015 - regulation of transforming growth factor beta receptor signaling pathway GO:0045892 - negative regulation of DNA-templated transcription GO:0070410 - co-SMAD binding and 1 others more... GO:0070412 - R-SMAD binding
Homologous interactions	11

Homologous interactions

Color coding for hit scores

	1 467 Hs			1 1127
+	Hs
		950328 SMAD3_HUMAN (1-467): 0: 617: 83.94		TRI33_HUMAN (1-1127): 0: 679: 100.00
+	Hs
		1035634 SMAD4_HUMAN (11-456): 4.53e-151: 443: 31.58		TRI33_HUMAN (1-1127): 0: 679: 100.00
+	Hs
		170332 SMAD3_HUMAN (1-467): 0: 617: 83.94		TIF1A_HUMAN (106-1127): 7.13e-146: 467: 44.94
+	Xl
		1122749 Q9W639_XENLA (11-456): 1.34e-149: 439: 31.93		TRI33_XENLA (53-1127): 0: 581: 79.50
+	Hs
		668718 SMAD2_HUMAN (1-467): 0: 675: 100.00		PML_HUMAN (83-635): 9.83e-61: 226: 17.01
+	Hs
		1078605 SMAD2_HUMAN (1-467): 0: 675: 100.00		CBP_HUMAN (517-1061): 4.54e-59: 225: 15.06
+	Hs
		668593 SMAD3_HUMAN (1-467): 0: 617: 83.94		PML_HUMAN (83-635): 9.83e-61: 226: 17.01
+	Hs
		950234 SMAD3_HUMAN (1-467): 0: 617: 83.94		CBP_HUMAN (517-1061): 4.54e-59: 225: 15.06
+	Hs
		1078735 SMAD1_HUMAN (1-467): 0: 565: 55.53		CBP_HUMAN (517-1061): 4.54e-59: 225: 15.06
+	Hs
		1029463 SMAD2_HUMAN (1-467): 0: 675: 100.00		TIF1B_HUMAN (631-1105): 6.36e-25: 114: 18.20
+	Hs
		1035526 SMAD4_HUMAN (11-456): 4.53e-151: 443: 31.58		CBP_HUMAN (517-1061): 4.54e-59: 225: 15.06