HitPredict

Interactor 1: SMAD1 (Q15797)
Mothers against decapentaplegic homolog 1 (MAD homolog 1) (Mothers against DPP homolog 1) (JV4-1) (Mad-related protein 1) (SMAD family member 1) (SMAD 1) (Smad1) (hSMAD1) (Transforming growth factor-beta-signaling protein 1) (BSP-1)
Gene: SMAD1

Interactor 2: PIAS4 (Q8N2W9)
E3 SUMO-protein ligase PIAS4 (EC 2.3.2.27) (PIASy) (Protein inhibitor of activated STAT protein 4) (Protein inhibitor of activated STAT protein gamma) (PIAS-gamma)
Gene: PIAS4

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.65	0.60	0.627

Property	Details
Experiments	12904571, 15231748
Identification Methods	affinity chromatography technology, two hybrid
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0006357 - regulation of transcription by RNA polymerase II GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 465 Hs			1 510
+	Hs
		950220 SMAD3_HUMAN (3-465): 0: 556: 59.66		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Hs
		1078585 SMAD2_HUMAN (3-465): 0: 541: 55.14		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Hs
		278098 SMAD1_HUMAN (1-465): 0: 658: 100.00		PIAS1_HUMAN (3-508): 2.66e-180: 522: 46.42
+	Hs
		1035500 SMAD4_HUMAN (13-454): 1.6e-150: 441: 34.28		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Hs
		950349 SMAD3_HUMAN (3-465): 0: 556: 59.66		PIAS3_HUMAN (3-509): 0: 540: 46.59
+	Hs
		1078712 SMAD2_HUMAN (3-465): 0: 541: 55.14		PIAS3_HUMAN (3-509): 0: 540: 46.59
+	Hs
		278264 SMAD3_HUMAN (3-465): 0: 556: 59.66		PIAS2_HUMAN (3-504): 9.32e-180: 520: 44.74
+	Hs
		1035653 SMAD4_HUMAN (13-454): 1.6e-150: 441: 34.28		PIAS3_HUMAN (3-509): 0: 540: 46.59
+	Hs
		278075 SMAD4_HUMAN (13-454): 1.6e-150: 441: 34.28		PIAS1_HUMAN (3-508): 2.66e-180: 522: 46.42
+	Hs
		206968 SMAD6_HUMAN (36-443): 2.87e-93: 292: 25.61		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Hs
		278278 SMAD4_HUMAN (13-454): 1.6e-150: 441: 34.28		PIAS2_HUMAN (3-504): 9.32e-180: 520: 44.74
+	Hs
		206981 SMAD6_HUMAN (36-443): 2.87e-93: 292: 25.61		PIAS3_HUMAN (3-509): 0: 540: 46.59
+	Hs
		950322 SMAD3_HUMAN (3-465): 0: 556: 59.66		ZMIZ1_HUMAN (73-419): 2.68e-71: 247: 23.42
+	Hs
		167374 SMAD7_HUMAN (30-131): 2.89e-25: 109: 32.46		PIAS4_HUMAN (1-510): 0: 670: 100.00
+	Hs
		1035630 SMAD4_HUMAN (13-454): 1.6e-150: 441: 34.28		ZMIZ1_HUMAN (73-419): 2.68e-71: 247: 23.42
+	Hs
		167316 SMAD7_HUMAN (30-131): 2.89e-25: 109: 32.46		PIAS1_HUMAN (3-508): 2.66e-180: 522: 46.42