HitPredict

Interactor 1: NEDD8 (Q15843)
NEDD8 (Neddylin) (Neural precursor cell expressed developmentally down-regulated protein 8) (NEDD-8) (Ubiquitin-like protein Nedd8)
Gene: NEDD8

Interactor 2: UBA3 (Q8TBC4)
NEDD8-activating enzyme E1 catalytic subunit (EC 6.2.1.64) (NEDD8-activating enzyme E1C) (Ubiquitin-activating enzyme E1C) (Ubiquitin-like modifier-activating enzyme 3) (Ubiquitin-activating enzyme 3)
Gene: UBA3

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.79	1.00	0.890

Property	Details
Experiments	27182664, 16620772, 16503656, 21145461
Identification Methods	enzymatic study, affinity chromatography technology, tandem affinity purification
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	ThiF~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0006508 - proteolysis GO:0036211 - protein modification process GO:0045116 - protein neddylation
Homologous interactions	6

Homologous interactions

Color coding for hit scores

	1 81 Hs			1 463
+	Hs
		1039423 NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00		ULA1_HUMAN (48-448): 1.5e-99: 310: 18.16
+	Hs
		1079620 NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00		SAE2_HUMAN (57-421): 9.94e-90: 287: 28.75
+	At
		790154 RUB1_ARATH (3-76): 7.75e-26: 94.5: 85.14		AXR1_ARATH (30-438): 7.34e-81: 262: 13.94
+	Sc
		977848 RUB1_YEAST (1-76): 1.45e-21: 81.3: 59.21		UBA3_YEAST (69-373): 3.09e-81: 255: 41.80
+	Hs
		607051 RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14		UBA1_HUMAN (46-363): 1.38e-28: 121: 15.25
+	Sc
		408274 RS31_YEAST (1-70): 3.03e-30: 105: 52.86		UBA1_YEAST (35-212): 5.24e-25: 110: 22.35