HitPredict

Interactor 1: NEDD8 (Q15843)
NEDD8 (Neddylin) (Neural precursor cell expressed developmentally down-regulated protein 8) (NEDD-8) (Ubiquitin-like protein Nedd8)
Gene: NEDD8

Interactor 2: CUL5 (Q93034)
Cullin-5 (CUL-5) (Vasopressin-activated calcium-mobilizing receptor 1) (VACM-1)
Gene: CUL5

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.89	1.00	0.945

Property	Details
Experiments	28514442, 28522566, 31253590, 33961781, 21145461, 18274552
Identification Methods	affinity chromatography technology, tandem affinity purification, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Cullin~ubiquitin, Cullin_Nedd8~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 81 Hs			1 780
+	Hs
		923983 RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14		CUL3_HUMAN (8-780): 0: 754: 22.96
+	Hs
		923984 RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14		CUL4A_HUMAN (16-780): 0: 744: 24.48
+	Hs
		923985 RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14		CUL4B_HUMAN (16-780): 0: 736: 25.39
+	Hs
		1041658 NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00		CUL3_HUMAN (8-780): 0: 754: 22.96
+	Hs
		924296 RL40_HUMAN (1-76): 3.79e-33: 112: 57.90		CUL4A_HUMAN (16-780): 0: 744: 24.48
+	At
		1352191 RUB1_ARATH (3-76): 7.75e-26: 94.5: 85.14		CUL3A_ARATH (16-780): 0: 759: 23.18
+	Hs
		1041004 NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00		CUL1_HUMAN (11-780): 0: 748: 27.84
+	Dm
		1213846 NEDD8_DROME (1-76): 2.21e-34: 113: 93.42		Q8IP45_DROME (5-780): 0: 736: 22.73
+	Hs
		1043010 NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00		CUL4A_HUMAN (16-780): 0: 744: 24.48
+	Hs
		475024 UBB_HUMAN (1-76): 2.4e-30: 107: 57.90		CUL4B_HUMAN (16-780): 0: 736: 25.39
+	Hs
		1043371 NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00		CUL4B_HUMAN (16-780): 0: 736: 25.39
+	At
		1321116 RUB1_ARATH (3-76): 7.75e-26: 94.5: 85.14		CUL3B_ARATH (16-780): 0: 744: 23.18
+	At
		1277343 RUB1_ARATH (3-76): 7.75e-26: 94.5: 85.14		CUL1_ARATH (8-780): 0: 721: 24.34
+	At
		1247030 RUB2_ARATH (3-76): 1.03e-24: 91.8: 85.14		CUL1_ARATH (8-780): 0: 721: 24.34
+	Dm
		1097203 NEDD8_DROME (1-76): 2.21e-34: 113: 93.42		CUL1_DROME (3-780): 0: 694: 25.99
+	Hs
		1041337 NEDD8_HUMAN (1-81): 2.07e-30: 103: 100.00		CUL2_HUMAN (6-780): 0: 699: 26.36
+	Sp
		142907 UBL1_SCHPO (1-76): 9.64e-30: 102: 81.58		CUL1_SCHPO (3-780): 0: 680: 24.97
+	Ce
		1088883 NEDD8_CAEEL (1-77): 7.56e-34: 112: 87.01		CUL3_CAEEL (17-780): 0: 658: 21.47
+	Sp
		145590 UBL1_SCHPO (1-76): 9.64e-30: 102: 81.58		CUL3_SCHPO (9-780): 0: 612: 20.78
+	Sc
		977843 RUB1_YEAST (1-76): 1.45e-21: 81.3: 59.21		CDC53_YEAST (6-780): 0: 600: 21.43