HitPredict

Interactor 1: NEDD8 (Q15843)
NEDD8 (Neddylin) (Neural precursor cell expressed developmentally down-regulated protein 8) (NEDD-8) (Ubiquitin-like protein Nedd8)
Gene: NEDD8

Interactor 2: UBP25 (Q9UHP3)
Ubiquitin carboxyl-terminal hydrolase 25 (EC 3.4.19.12) (Deubiquitinating enzyme 25) (USP on chromosome 21) (Ubiquitin thioesterase 25) (Ubiquitin-specific-processing protease 25)
Gene: USP25

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.776

Property	Details
Experiments	28619731, 19615732
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association

Property	Details
Interacting Pfam domains	UCH~ubiquitin, UIM~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0006508 - proteolysis GO:0031625 - ubiquitin protein ligase binding GO:0036211 - protein modification process
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 81 Hs			1 1055
+	Hs
		475225 UBB_HUMAN (1-76): 2.4e-30: 107: 57.90		UBP25_HUMAN (1-1055): 0: 1021: 100.00
+	Hs
		800571 RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14		UBP5_HUMAN (148-656): 1.28e-85: 297: 15.09
+	Hs
		474951 UBB_HUMAN (1-76): 2.4e-30: 107: 57.90		UBP5_HUMAN (148-656): 1.28e-85: 297: 15.09
+	Hs
		905374 RL40_HUMAN (1-76): 3.79e-33: 112: 57.90		UBP46_HUMAN (159-470): 6.89e-87: 286: 16.86
+	Rn
		94336 RL40_RAT (1-76): 3.79e-33: 112: 57.90		UBP46_RAT (159-470): 3.88e-87: 286: 16.86
+	Hs
		474982 UBB_HUMAN (1-76): 2.4e-30: 107: 57.90		UBP46_HUMAN (159-470): 6.89e-87: 286: 16.86
+	Hs
		924100 RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14		USP9X_HUMAN (142-459): 2.23e-67: 250: 20.22
+	Hs
		924372 RL40_HUMAN (1-76): 3.79e-33: 112: 57.90		USP9X_HUMAN (142-459): 2.23e-67: 250: 20.22
+	Hs
		475082 UBB_HUMAN (1-76): 2.4e-30: 107: 57.90		UBP48_HUMAN (157-406): 1.37e-66: 245: 25.86
+	Mm
		476669 UBB_MOUSE (1-76): 1.5e-29: 107: 57.90		UBP48_MOUSE (157-406): 1.11e-64: 240: 25.86
+	At
		1114637 RL40B_ARATH (1-76): 1.27e-31: 108: 56.58		UBP15_ARATH (467-802): 2.83e-32: 137: 11.50
+	Hs
		924373 RL40_HUMAN (1-76): 3.79e-33: 112: 57.90		UBP7_HUMAN (547-758): 1.65e-17: 90.2: 21.78
+	Hs
		475123 UBB_HUMAN (1-76): 2.4e-30: 107: 57.90		UBP7_HUMAN (547-758): 1.65e-17: 90.2: 21.78
+	Hs
		924201 RS27A_HUMAN (1-77): 6.66e-37: 122: 57.14		UBP36_HUMAN (560-684): 1.95e-11: 70.7: 22.84
+	Hs
		271114 Q5U5U6_HUMAN (1-76): 2.4e-30: 107: 57.90		UBP2_HUMAN (514-660): 9.45e-16: 83.8: 17.69
+	At
		1114640 RL40B_ARATH (1-76): 1.27e-31: 108: 56.58		UBP16_ARATH (478-808): 1.78e-12: 73.8: 12.87
+	Dm
		1099208 R9PY16_DROME (1-68): 1.24e-24: 94.8: 51.47		UBPE_DROME (557-685): 6.65e-10: 65.6: 31.21