HitPredict

Interactor 1: CPSF6 (Q16630)
Cleavage and polyadenylation specificity factor subunit 6 (Cleavage and polyadenylation specificity factor 68 kDa subunit) (CPSF 68 kDa subunit) (Cleavage factor Im complex 68 kDa subunit) (CFIm68) (Pre-mRNA cleavage factor Im 68 kDa subunit) (Protein HPBRII-4/7)
Gene: CPSF6

Interactor 2: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.82	0.60	0.703

Property	Details
Experiments	28514442, 35271311, 16055720, 26508657
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0016020 - membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 551 Hs			1 903
+	Hs
		1084614 CPSF6_HUMAN (1-520): 3.7e-92: 294: 100.00		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125492 CPSF6_HUMAN (1-520): 3.7e-92: 294: 100.00		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		1235773 CPSF7_HUMAN (1-525): 3.55e-48: 175: 40.82		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Hs
		516643 PABP1_HUMAN (81-518): 2.46e-24: 109: 16.40		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Hs
		1235798 CPSF7_HUMAN (1-525): 3.55e-48: 175: 40.82		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125259 SMCA2_HUMAN (179-551): 2.13e-47: 180: 14.93		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		125581 CPSF7_HUMAN (1-525): 3.55e-48: 175: 40.82		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		125260 SMCA4_HUMAN (126-551): 8.83e-45: 172: 15.98		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		808144 CPSF6_HUMAN (1-520): 3.7e-92: 294: 100.00		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		125430 PABP4_HUMAN (81-517): 7.23e-31: 129: 13.51		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		125122 PABP1_HUMAN (81-518): 2.46e-24: 109: 16.40		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		1311993 DIDO1_HUMAN (164-416): 4.81e-09: 61.5: 21.59		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125274 SC24C_HUMAN (233-440): 1.79e-12: 72.2: 17.05		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		125061 SC24D_HUMAN (131-353): 2.02e-11: 68.8: 21.49		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Mm
		1208066 DIDO1_MOUSE (151-446): 1.18e-09: 63.3: 17.16		WWP2_MOUSE (1-903): 0: 820: 54.52
+	Hs
		125687 DIDO1_HUMAN (164-416): 4.81e-09: 61.5: 21.59		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Sc
		376913 PABP_YEAST (82-174): 3.01e-12: 71.1: 27.96		TOM1_YEAST (487-902): 5.57e-156: 510: 39.10
+	Hs
		1084629 CPSF6_HUMAN (1-520): 3.7e-92: 294: 100.00		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		846873 SMCA2_HUMAN (179-551): 2.13e-47: 180: 14.93		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Hs
		1312007 DIDO1_HUMAN (164-416): 4.81e-09: 61.5: 21.59		HECW2_HUMAN (1-512): 1.38e-13: 77.2: 11.34