HitPredict

Interactor 1: HIF1A (Q16665)
Hypoxia-inducible factor 1-alpha (HIF-1-alpha) (HIF1-alpha) (ARNT-interacting protein) (Basic-helix-loop-helix-PAS protein MOP1) (Class E basic helix-loop-helix protein 78) (bHLHe78) (Member of PAS protein 1) (PAS domain-containing protein 8)
Gene: HIF1A

Interactor 2: MED12 (Q93074)
Mediator of RNA polymerase II transcription subunit 12 (Activator-recruited cofactor 240 kDa component) (ARC240) (CAG repeat protein 45) (Mediator complex subunit 12) (OPA-containing protein) (Thyroid hormone receptor-associated protein complex 230 kDa component) (Trap230) (Trinucleotide repeat-containing gene 11 protein)
Gene: MED12

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.47	0.60	0.536

Property	Details
Experiments	23746844
Identification Methods	affinity chromatography technology, pull down
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0045893 - positive regulation of DNA-templated transcription GO:0045944 - positive regulation of transcription by RNA polymerase II
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 826 Hs			1 2177
+	Hs
		1274337 EPAS1_HUMAN (4-824): 5.7e-171: 518: 41.24		MED12_HUMAN (1-2177): 0: 2488: 100.00
+	Hs
		1085623 HIF1A_HUMAN (1-826): 0: 741: 100.00		CBP_HUMAN (1582-2176): 2.69e-11: 71.5: 13.45
+	Hs
		1085646 HIF1A_HUMAN (1-826): 0: 741: 100.00		EP400_HUMAN (1812-2174): 7.89e-09: 63.4: 17.63
+	Rn
		193481 HIF1A_RAT (1-826): 0: 714: 89.40		CBP_RAT (1658-2026): 7.81e-09: 63.4: 11.32
+	Mm
		70923 HIF1A_MOUSE (1-826): 0: 706: 87.63		EP300_MOUSE (1845-2169): 2.28e-10: 68.7: 22.10
+	Hs
		1270775 EPAS1_HUMAN (4-824): 5.7e-171: 518: 41.24		CBP_HUMAN (1582-2176): 2.69e-11: 71.5: 13.45
+	Hs
		1244293 EPAS1_HUMAN (4-824): 5.7e-171: 518: 41.24		ARI1B_HUMAN (1839-2172): 5.1e-10: 67.3: 14.20
+	Hs
		1075384 NCOA2_HUMAN (1-747): 3.97e-109: 367: 15.62		CBP_HUMAN (1582-2176): 2.69e-11: 71.5: 13.45
+	Hs
		1270774 NPAS2_HUMAN (5-749): 1.34e-113: 367: 18.32		CBP_HUMAN (1582-2176): 2.69e-11: 71.5: 13.45
+	Mm
		70944 BMAL1_MOUSE (7-505): 2.8e-116: 368: 20.92		EP300_MOUSE (1845-2169): 2.28e-10: 68.7: 22.10
+	Mm
		799605 BMAL1_MOUSE (7-505): 2.8e-116: 368: 20.92		CBP_MOUSE (1658-2031): 7.06e-10: 66.9: 11.66
+	Hs
		1057760 NCOA2_HUMAN (1-747): 3.97e-109: 367: 15.62		NCOA6_HUMAN (1716-2173): 1.71e-09: 65.7: 11.91
+	Hs
		126156 BMAL1_HUMAN (7-505): 9.03e-114: 361: 20.88		CBP_HUMAN (1582-2176): 2.69e-11: 71.5: 13.45
+	Ce
		108089 HIF1_CAEEL (8-535): 5.37e-95: 314: 23.28		UNC89_CAEEL (1599-2149): 1.94e-20: 101: 10.83
+	Hs
		181549 CLOCK_HUMAN (2-696): 1.68e-102: 338: 18.24		CBP_HUMAN (1582-2176): 2.69e-11: 71.5: 13.45
+	Hs
		1270854 NCOA3_HUMAN (1-746): 4.42e-98: 335: 15.31		CBP_HUMAN (1582-2176): 2.69e-11: 71.5: 13.45
+	Mm
		101411 CLOCK_MOUSE (2-704): 2.25e-102: 338: 17.88		F8VPR5_MOUSE (1658-2031): 7.3e-10: 66.9: 11.66
+	Mm
		70918 BMAL2_MOUSE (13-508): 3.25e-100: 324: 20.86		EP300_MOUSE (1845-2169): 2.28e-10: 68.7: 22.10
+	Mm
		799600 BMAL2_MOUSE (13-508): 3.25e-100: 324: 20.86		CBP_MOUSE (1658-2031): 7.06e-10: 66.9: 11.66