HitPredict

Interactor 1: RAC4 (Q38919)
Rac-like GTP-binding protein ARAC4 (AtRAC4) (GTPase protein ROP2)
Gene: ARAC4

Interactor 2: SPK1 (Q8SAB7)
Guanine nucleotide exchange factor SPIKE 1
Gene: SPK1

Species: Arabidopsis thaliana

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.68	1.00	0.831

Property	Details
Experiments	18308939, 17267444
Identification Methods	affinity chromatography technology, pull down, two hybrid, enzymatic study
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	DHR-2_Lobe_B~Ras, DHR-2_Lobe_C~Ras
Common GO terms	GO:0005634 - nucleus GO:0007264 - small GTPase-mediated signal transduction GO:0008360 - regulation of cell shape GO:0043622 - cortical microtubule organization GO:0048446 - petal morphogenesis and 1 others more... GO:0051211 - anisotropic cell growth
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 195 At			1 1830
+	At
		287603 RAC10_ARATH (2-179): 1.53e-82: 245: 81.46		SPK1_ARATH (1-1830): 0: 2088: 100.00
+	At
		1248269 RAC9_ARATH (6-195): 3.3e-78: 234: 77.49		SPK1_ARATH (1-1830): 0: 2088: 100.00
+	At
		1105166 RAC5_ARATH (2-195): 1.84e-76: 229: 97.94		SPK1_ARATH (1-1830): 0: 2088: 100.00
+	Hs
		925332 RAC1_HUMAN (6-195): 3.93e-81: 240: 61.98		DOC11_HUMAN (77-1827): 0: 1614: 21.33
+	Hs
		545421 RAC2_HUMAN (6-195): 3.03e-77: 231: 60.94		DOC11_HUMAN (77-1827): 0: 1614: 21.33
+	Hs
		925588 RAC1_HUMAN (6-195): 3.93e-81: 240: 61.98		DOCK9_HUMAN (46-1827): 0: 1537: 19.31
+	Hs
		545596 RAC2_HUMAN (6-195): 3.03e-77: 231: 60.94		DOCK9_HUMAN (46-1827): 0: 1537: 19.31
+	Hs
		925518 RAC1_HUMAN (6-195): 3.93e-81: 240: 61.98		DOCK6_HUMAN (16-979): 0: 755: 20.07
+	Hs
		925526 RAC1_HUMAN (6-195): 3.93e-81: 240: 61.98		DOCK7_HUMAN (14-731): 0: 656: 21.52
+	Hs
		925448 RAC1_HUMAN (6-195): 3.93e-81: 240: 61.98		DOCK8_HUMAN (14-731): 0: 602: 20.24
+	Hs
		925429 RAC1_HUMAN (6-195): 3.93e-81: 240: 61.98		DOCK4_HUMAN (463-1026): 7.18e-139: 479: 16.22
+	Dm
		776892 RAC1_DROME (6-195): 4e-78: 233: 60.42		Q8MQW7_DROME (1270-1830): 2.47e-131: 446: 15.17
+	Hs
		925425 RAC1_HUMAN (6-195): 3.93e-81: 240: 61.98		DOCK3_HUMAN (463-1026): 4.99e-70: 264: 15.18
+	Mm
		925807 RAC1_MOUSE (6-195): 3.93e-81: 240: 61.98		DOCK1_MOUSE (463-1017): 1.06e-47: 190: 15.15
+	Hs
		925620 RAC1_HUMAN (6-195): 3.93e-81: 240: 61.98		DOCK5_HUMAN (463-1026): 4.43e-47: 188: 14.88
+	Hs
		925276 RAC1_HUMAN (6-195): 3.93e-81: 240: 61.98		DOCK1_HUMAN (463-1017): 1.06e-45: 184: 14.96
+	Hs
		545622 RAC2_HUMAN (6-195): 3.03e-77: 231: 60.94		DOCK5_HUMAN (463-1026): 4.43e-47: 188: 14.88
+	Hs
		925486 RAC1_HUMAN (6-195): 3.93e-81: 240: 61.98		DOCK2_HUMAN (463-1026): 1.04e-43: 177: 13.46
+	Hs
		545372 RAC2_HUMAN (6-195): 3.03e-77: 231: 60.94		DOCK1_HUMAN (463-1017): 1.06e-45: 184: 14.96
+	Hs
		545535 RAC2_HUMAN (6-195): 3.03e-77: 231: 60.94		DOCK2_HUMAN (463-1026): 1.04e-43: 177: 13.46