HitPredict

Interactor 1: ASCL4 (Q6XD76)
Achaete-scute homolog 4 (ASH-4) (hASH4) (Achaete-scute-like protein 4) (Class A basic helix-loop-helix protein 44) (bHLHa44)
Gene: ASCL4

Interactor 2: HTF4 (Q99081)
Transcription factor 12 (TCF-12) (Class B basic helix-loop-helix protein 20) (bHLHb20) (DNA-binding protein HTF4) (E-box-binding protein) (Transcription factor HTF-4)
Gene: TCF12

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.86	1.00	0.933

Property	Details
Experiments	25416956, 14516699, 32296183, 33961781, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, two hybrid prey pooling approach, two hybrid array, validated two hybrid, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	HLH~HLH
Common GO terms	GO:0000785 - chromatin GO:0000981 - DNA-binding transcription factor activity, RNA polymerase II-specific GO:0006357 - regulation of transcription by RNA polymerase II GO:0090575 - RNA polymerase II transcription regulator complex
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 172 Hs			1 682
+	Hs
		840637 ASCL1_HUMAN (16-170): 7.3e-31: 113: 34.59		HTF4_HUMAN (1-682): 0: 921: 100.00
+	Hs
		899129 HAND2_HUMAN (6-171): 3.49e-30: 111: 21.18		HTF4_HUMAN (1-682): 0: 921: 100.00
+	Mm
		968766 ASCL1_MOUSE (33-170): 5.95e-31: 113: 34.29		HTF4_MOUSE (1-682): 0: 907: 92.21
+	Mm
		1142072 HAND2_MOUSE (6-171): 3.49e-30: 111: 21.18		HTF4_MOUSE (1-682): 0: 907: 92.21
+	Mm
		1141281 HAND2_MOUSE (6-171): 3.49e-30: 111: 21.18		ITF2_MOUSE (2-682): 0: 804: 62.82
+	Hs
		551181 ASCL4_HUMAN (1-156): 7.3e-41: 137: 100.00		ITF2_HUMAN (2-682): 0: 777: 62.01
+	Hs
		551272 NGN2_HUMAN (1-172): 8.03e-31: 114: 22.78		ITF2_HUMAN (2-682): 0: 777: 62.01
+	Hs
		551093 ASCL1_HUMAN (16-170): 7.3e-31: 113: 34.59		ITF2_HUMAN (2-682): 0: 777: 62.01
+	Hs
		551542 ASCL4_HUMAN (1-156): 7.3e-41: 137: 100.00		TFE2_HUMAN (2-682): 0: 753: 48.93
+	Hs
		551184 PTF1A_HUMAN (19-171): 9.47e-30: 112: 26.35		ITF2_HUMAN (2-682): 0: 777: 62.01
+	Hs
		551106 HAND2_HUMAN (6-171): 3.49e-30: 111: 21.18		ITF2_HUMAN (2-682): 0: 777: 62.01
+	Hs
		551220 TWST2_HUMAN (22-167): 3.67e-30: 109: 21.23		ITF2_HUMAN (2-682): 0: 777: 62.01
+	Hs
		551486 ASCL1_HUMAN (16-170): 7.3e-31: 113: 34.59		TFE2_HUMAN (2-682): 0: 753: 48.93
+	Hs
		551544 PTF1A_HUMAN (19-171): 9.47e-30: 112: 26.35		TFE2_HUMAN (2-682): 0: 753: 48.93
+	Hs
		551490 HAND2_HUMAN (6-171): 3.49e-30: 111: 21.18		TFE2_HUMAN (2-682): 0: 753: 48.93
+	Hs
		551559 TWST2_HUMAN (22-167): 3.67e-30: 109: 21.23		TFE2_HUMAN (2-682): 0: 753: 48.93
+	Mm
		485935 MYOD1_MOUSE (5-166): 6.04e-30: 113: 28.14		TFE2_MOUSE (1-682): 0: 732: 49.43
+	Mm
		550202 ASCL1_MOUSE (33-170): 5.95e-31: 113: 34.29		TFE2_MOUSE (1-682): 0: 732: 49.43
+	Mm
		550211 HAND2_MOUSE (6-171): 3.49e-30: 111: 21.18		TFE2_MOUSE (1-682): 0: 732: 49.43
+	Dm
		80865 DIMM_DROME (19-167): 1.71e-29: 113: 22.82		DA_DROME (272-679): 1.33e-107: 343: 36.92