HitPredict

Interactor 1: HUWE1 (Q7Z6Z7)
E3 ubiquitin-protein ligase HUWE1 (EC 2.3.2.26) (ARF-binding protein 1) (ARF-BP1) (HECT, UBA and WWE domain-containing protein 1) (HECT-type E3 ubiquitin transferase HUWE1) (Homologous to E6AP carboxyl terminus homologous protein 9) (HectH9) (Large structure of UREB1) (LASU1) (Mcl-1 ubiquitin ligase E3) (Mule) (Upstream regulatory element-binding protein 1) (URE-B1) (URE-binding protein 1)
Gene: HUWE1

Interactor 2: ARRD4 (Q8NCT1)
Arrestin domain-containing protein 4
Gene: ARRDC4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	0.60	0.536

Property	Details
Experiments	38270169
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 4374 Hs			1 418
+	Hs
		1192131 HUWE1_HUMAN (1-4374): 0: 3240: 100.00		ARRD2_HUMAN (16-405): 2.62e-123: 365: 46.19
+	Hs
		1192211 HUWE1_HUMAN (1-4374): 0: 3240: 100.00		TXNIP_HUMAN (16-403): 6.64e-116: 345: 43.45
+	Hs
		1241904 ITCH_HUMAN (3507-4372): 0: 672: 23.10		ARRD4_HUMAN (1-418): 3.79e-150: 433: 100.00
+	Hs
		1285047 ITCH_HUMAN (3507-4372): 0: 672: 23.10		ARRD3_HUMAN (15-413): 6.19e-140: 407: 51.35
+	Hs
		125586 WWP2_HUMAN (3506-4372): 0: 624: 24.57		ARRD4_HUMAN (1-418): 3.79e-150: 433: 100.00
+	Hs
		1252807 ITCH_HUMAN (3507-4372): 0: 672: 23.10		ARRD2_HUMAN (16-405): 2.62e-123: 365: 46.19
+	Hs
		125633 WWP2_HUMAN (3506-4372): 0: 624: 24.57		ARRD3_HUMAN (15-413): 6.19e-140: 407: 51.35
+	Hs
		1294858 ITCH_HUMAN (3507-4372): 0: 672: 23.10		TXNIP_HUMAN (16-403): 6.64e-116: 345: 43.45
+	Hs
		125595 WWP2_HUMAN (3506-4372): 0: 624: 24.57		ARRD2_HUMAN (16-405): 2.62e-123: 365: 46.19
+	Hs
		125707 WWP2_HUMAN (3506-4372): 0: 624: 24.57		TXNIP_HUMAN (16-403): 6.64e-116: 345: 43.45
+	Dm
		1367634 SUDX_DROME (3526-4371): 0: 636: 23.22		Q9VI00_DROME (18-415): 2.18e-96: 297: 23.94
+	Mm
		1208049 ITCH_MOUSE (3507-4372): 0: 685: 24.20		ARRB2_MOUSE (24-363): 1.54e-76: 245: 14.82
+	Hs
		686035 ITCH_HUMAN (3507-4372): 0: 672: 23.10		ARRB2_HUMAN (24-363): 9.37e-79: 250: 14.32
+	Hs
		827260 ITCH_HUMAN (3507-4372): 0: 672: 23.10		ARRB1_HUMAN (24-339): 9.17e-78: 248: 14.40
+	Dm
		1367613 SUDX_DROME (3526-4371): 0: 636: 23.22		Q9VHZ7_DROME (20-320): 5.91e-81: 259: 25.33
+	Hs
		1234600 ITCH_HUMAN (3507-4372): 0: 672: 23.10		ARRD1_HUMAN (15-405): 5.14e-66: 218: 23.86
+	Dm
		1366476 SUDX_DROME (3526-4371): 0: 636: 23.22		Q9VGU4_DROME (19-313): 1.36e-61: 209: 20.13
+	Hs
		125580 WWP2_HUMAN (3506-4372): 0: 624: 24.57		ARRD1_HUMAN (15-405): 5.14e-66: 218: 23.86
+	Hs
		1234609 SMUF1_HUMAN (3772-4371): 0: 620: 32.84		ARRD1_HUMAN (15-405): 5.14e-66: 218: 23.86
+	Hs
		1234602 NED4L_HUMAN (3542-4372): 0: 618: 27.53		ARRD1_HUMAN (15-405): 5.14e-66: 218: 23.86