HitPredict

Interactor 1: PIF1 (Q8GZM7)
Transcription factor PIF1 (Basic helix-loop-helix protein 15) (AtbHLH15) (bHLH 15) (Phytochrome-interacting factor 1) (Protein PHYTOCHROME INTERACTING FACTOR 3-LIKE 5) (Transcription factor EN 101) (bHLH transcription factor bHLH015)
Gene: PIF1

Interactor 2: HFR1 (Q9FE22)
Transcription factor HFR1 (Basic helix-loop-helix protein 26) (AtbHLH26) (bHLH 26) (Protein LONG HYPOCOTYL IN FAR-RED 1) (Protein REDUCED PHYTOCHROME SIGNALING) (Reduced sensitivity to far-red light) (Transcription factor EN 68) (bHLH transcription factor bHLH026)
Gene: HFR1

Species: Arabidopsis thaliana

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.67	1.00	0.821

Property	Details
Experiments	21928113, 24179122
Identification Methods	two hybrid, protein complementation assay, affinity chromatography technology
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	HLH~HLH
Common GO terms	GO:0003700 - DNA-binding transcription factor activity GO:0005634 - nucleus GO:0009408 - response to heat GO:0009737 - response to abscisic acid GO:0046983 - protein dimerization activity
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 478 At			1 292
+	At
		1210191 PIF1_ARATH (1-478): 8.72e-90: 283: 100.00		PIF1_ARATH (82-248): 8.85e-31: 121: 28.74
+	At
		1195588 PIF5_ARATH (17-468): 5.9e-38: 145: 22.29		HFR1_ARATH (1-292): 3.63e-84: 256: 100.00
+	At
		1210193 PIF1_ARATH (1-478): 8.72e-90: 283: 100.00		PIF6_ARATH (50-275): 4.79e-29: 115: 21.09
+	At
		1226062 PIL1_ARATH (25-455): 1.19e-36: 141: 21.25		HFR1_ARATH (1-292): 3.63e-84: 256: 100.00
+	At
		285447 PIF1_ARATH (1-478): 8.72e-90: 283: 100.00		PIF3_ARATH (104-235): 2.98e-27: 112: 30.56
+	At
		1195586 PIF1_ARATH (1-478): 8.72e-90: 283: 100.00		PIF5_ARATH (109-237): 3.91e-26: 108: 28.00
+	At
		1210196 PIF1_ARATH (1-478): 8.72e-90: 283: 100.00		PIF4_ARATH (93-249): 1.49e-19: 89.9: 24.86
+	At
		1123026 PIF1_ARATH (1-478): 8.72e-90: 283: 100.00		PIF7_ARATH (96-206): 2.77e-12: 68.3: 28.83
+	At
		1209823 BH063_ARATH (144-441): 2.09e-37: 141: 20.78		BEE2_ARATH (50-275): 5.72e-36: 132: 19.74
+	At
		1209825 BH063_ARATH (144-441): 2.09e-37: 141: 20.78		BH076_ARATH (14-249): 1.24e-34: 130: 18.14
+	At
		1209838 BH063_ARATH (144-441): 2.09e-37: 141: 20.78		BH079_ARATH (35-206): 4.33e-33: 124: 20.23
+	At
		1209850 BH063_ARATH (144-441): 2.09e-37: 141: 20.78		BH062_ARATH (50-285): 1.95e-29: 117: 15.61
+	At
		1209831 BH063_ARATH (144-441): 2.09e-37: 141: 20.78		BH078_ARATH (63-285): 1.15e-28: 116: 17.26
+	At
		285443 PIF5_ARATH (17-468): 5.9e-38: 145: 22.29		PIF3_ARATH (104-235): 2.98e-27: 112: 30.56
+	Zm
		85865 C0PHL6_MAIZE (69-447): 3.99e-35: 140: 14.75		C0PHL6_MAIZE (138-292): 6.09e-29: 117: 14.74
+	At
		1187165 LRL1_ARATH (147-477): 3.17e-38: 144: 16.92		BH153_ARATH (104-258): 7.63e-30: 111: 19.87
+	At
		1187142 LRL1_ARATH (147-477): 3.17e-38: 144: 16.92		BH154_ARATH (104-258): 6.16e-27: 104: 22.79
+	At
		1140937 PIL1_ARATH (25-455): 1.19e-36: 141: 21.25		BH109_ARATH (58-248): 3.87e-27: 105: 22.45
+	At
		1123027 PIL1_ARATH (25-455): 1.19e-36: 141: 21.25		PIF7_ARATH (96-206): 2.77e-12: 68.3: 28.83