HitPredict

Interactor 1: WWP2 (O00308)
NEDD4-like E3 ubiquitin-protein ligase WWP2 (EC 2.3.2.26) (Atrophin-1-interacting protein 2) (AIP2) (HECT-type E3 ubiquitin transferase WWP2) (WW domain-containing protein 2)
Gene: WWP2

Interactor 2: SMAD7 (O15105)
Mothers against decapentaplegic homolog 7 (MAD homolog 7) (Mothers against DPP homolog 7) (Mothers against decapentaplegic homolog 8) (MAD homolog 8) (Mothers against DPP homolog 8) (SMAD family member 7) (SMAD 7) (Smad7) (hSMAD7)
Gene: SMAD7

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.75	0.60	0.675

Property	Details
Experiments	21258410, 31546607, 22773947
Identification Methods	affinity chromatography technology, enzymatic study, x-ray crystallography, anti tag coimmunoprecipitation, pull down, anti bait coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000122 - negative regulation of transcription by RNA polymerase II GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 870 Hs			1 426
+	Hs
		125023 WWP2_HUMAN (1-870): 0: 982: 100.00		SMAD6_HUMAN (1-425): 5.75e-147: 428: 43.56
+	Hs
		125369 WWP2_HUMAN (1-870): 0: 982: 100.00		SMAD3_HUMAN (81-424): 2.22e-146: 424: 26.72
+	Hs
		167384 ITCH_HUMAN (1-870): 0: 902: 54.26		SMAD7_HUMAN (1-426): 1.58e-170: 486: 100.00
+	Hs
		125487 WWP2_HUMAN (1-870): 0: 982: 100.00		SMAD2_HUMAN (82-424): 5.2e-138: 404: 24.53
+	Hs
		167393 WWP1_HUMAN (1-870): 0: 888: 58.38		SMAD7_HUMAN (1-426): 1.58e-170: 486: 100.00
+	Hs
		206970 ITCH_HUMAN (1-870): 0: 902: 54.26		SMAD6_HUMAN (1-425): 5.75e-147: 428: 43.56
+	Hs
		950248 ITCH_HUMAN (1-870): 0: 902: 54.26		SMAD3_HUMAN (81-424): 2.22e-146: 424: 26.72
+	Hs
		206975 WWP1_HUMAN (1-870): 0: 888: 58.38		SMAD6_HUMAN (1-425): 5.75e-147: 428: 43.56
+	Hs
		1305881 WWP1_HUMAN (1-870): 0: 888: 58.38		SMAD5_HUMAN (79-424): 5.54e-146: 425: 24.08
+	Hs
		950278 WWP1_HUMAN (1-870): 0: 888: 58.38		SMAD3_HUMAN (81-424): 2.22e-146: 424: 26.72
+	Hs
		1078757 WWP1_HUMAN (1-870): 0: 888: 58.38		SMAD1_HUMAN (79-424): 2.02e-145: 423: 24.26
+	Hs
		1078624 ITCH_HUMAN (1-870): 0: 902: 54.26		SMAD2_HUMAN (82-424): 5.2e-138: 404: 24.53
+	Mm
		1143948 ITCH_MOUSE (1-870): 0: 892: 55.81		SMAD2_MOUSE (82-424): 1.68e-138: 406: 24.53
+	Hs
		1078654 WWP1_HUMAN (1-870): 0: 888: 58.38		SMAD2_HUMAN (82-424): 5.2e-138: 404: 24.53
+	Dm
		785516 SUDX_DROME (14-870): 0: 852: 52.14		MAD_DROME (72-424): 2.77e-151: 438: 25.48
+	Hs
		167385 NED4L_HUMAN (5-867): 0: 785: 32.17		SMAD7_HUMAN (1-426): 1.58e-170: 486: 100.00
+	Hs
		206971 NED4L_HUMAN (5-867): 0: 785: 32.17		SMAD6_HUMAN (1-425): 5.75e-147: 428: 43.56
+	Hs
		950249 NED4L_HUMAN (5-867): 0: 785: 32.17		SMAD3_HUMAN (81-424): 2.22e-146: 424: 26.72
+	Hs
		1035547 ITCH_HUMAN (1-870): 0: 902: 54.26		SMAD4_HUMAN (54-259): 3.55e-41: 155: 22.75
+	Hs
		1035583 WWP1_HUMAN (1-870): 0: 888: 58.38		SMAD4_HUMAN (54-259): 3.55e-41: 155: 22.75