HitPredict

Interactor 1: ATG4A (Q8WYN0)
Cysteine protease ATG4A (EC 3.4.22.-) (AUT-like 2 cysteine endopeptidase) (Autophagy-related cysteine endopeptidase 2) (Autophagin-2) (Autophagy-related protein 4 homolog A) (HsAPG4A) (hAPG4A)
Gene: ATG4A

Interactor 2: GBRL1 (Q9H0R8)
Gamma-aminobutyric acid receptor-associated protein-like 1 (Early estrogen-regulated protein) (GABA(A) receptor-associated protein-like 1) (Glandular epithelial cell protein 1) (GEC-1)
Gene: GABARAPL1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.75	1.00	0.871

Property	Details
Experiments	25416956, 32296183, unassigned1304
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	ATG8~Peptidase_C54
Common GO terms	GO:0000045 - autophagosome assembly
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 398 Hs			1 117
+	Hs
		891248 ATG4A_HUMAN (1-398): 0: 603: 100.00		GBRL2_HUMAN (1-116): 1.39e-57: 175: 61.21
+	Hs
		306253 ATG4A_HUMAN (1-398): 0: 603: 100.00		GBRAP_HUMAN (1-116): 2.96e-56: 171: 87.07
+	Hs
		1264550 ATG4A_HUMAN (1-398): 0: 603: 100.00		MLP3A_HUMAN (5-116): 1.09e-44: 142: 34.21
+	Hs
		1264548 ATG4A_HUMAN (1-398): 0: 603: 100.00		MLP3B_HUMAN (2-116): 1.06e-43: 140: 31.62
+	Hs
		891370 ATG4B_HUMAN (19-398): 0: 546: 54.64		GBRL2_HUMAN (1-116): 1.39e-57: 175: 61.21
+	Hs
		1328075 ATG4B_HUMAN (19-398): 0: 546: 54.64		GBRL1_HUMAN (1-117): 4.54e-56: 171: 100.00
+	Hs
		306368 ATG4B_HUMAN (19-398): 0: 546: 54.64		GBRAP_HUMAN (1-116): 2.96e-56: 171: 87.07
+	Mm
		1206409 ATG4B_MOUSE (19-398): 0: 548: 56.07		MLP3A_MOUSE (5-116): 1.09e-44: 142: 34.21
+	Hs
		1315816 ATG4B_HUMAN (19-398): 0: 546: 54.64		MLP3C_HUMAN (5-116): 1.61e-44: 143: 39.47
+	Hs
		1330686 ATG4B_HUMAN (19-398): 0: 546: 54.64		MLP3A_HUMAN (5-116): 1.09e-44: 142: 34.21
+	Hs
		1326231 ATG4B_HUMAN (19-398): 0: 546: 54.64		MLP3B_HUMAN (2-116): 1.06e-43: 140: 31.62
+	Hs
		61909 ATG4B_HUMAN (19-398): 0: 546: 54.64		MP3B2_HUMAN (2-116): 6.71e-43: 138: 30.77
+	Ce
		111920 ATG41_CAEEL (15-398): 6.27e-163: 467: 38.90		LGG1_CAEEL (1-116): 3.74e-55: 169: 78.45
+	Ce
		111921 ATG41_CAEEL (15-398): 6.27e-163: 467: 38.90		LGG2_CAEEL (4-116): 6.14e-39: 128: 35.65
+	Hs
		891203 ATG4D_HUMAN (27-358): 1.13e-137: 403: 31.10		GBRL2_HUMAN (1-116): 1.39e-57: 175: 61.21
+	Hs
		1198603 ATG4D_HUMAN (27-358): 1.13e-137: 403: 31.10		GBRL1_HUMAN (1-117): 4.54e-56: 171: 100.00
+	Hs
		306219 ATG4D_HUMAN (27-358): 1.13e-137: 403: 31.10		GBRAP_HUMAN (1-116): 2.96e-56: 171: 87.07
+	Dm
		1179054 Q9VPW2_DROME (20-392): 8.03e-135: 393: 40.58		Q7K1J3_DROME (1-117): 1.71e-54: 168: 73.50
+	Dm
		1364631 Q9VPW2_DROME (20-392): 8.03e-135: 393: 40.58		Q9VEG5_DROME (1-117): 1.86e-54: 167: 73.50
+	Hs
		1198602 ATG4D_HUMAN (27-358): 1.13e-137: 403: 31.10		MLP3B_HUMAN (2-116): 1.06e-43: 140: 31.62