HitPredict

Interactor 1: WASL (O00401)
Actin nucleation-promoting factor WASL (Neural Wiskott-Aldrich syndrome protein) (N-WASP)
Gene: WASL

Interactor 2: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.90	0.60	0.735

Property	Details
Experiments	11340081, 26496610, 26871637, 33961781, 16595635, 17474147
Identification Methods	far western blotting, affinity chromatography technology, two hybrid, pull down, peptide array, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0045944 - positive regulation of transcription by RNA polymerase II
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 505 Hs			1 377
+	Hs
		203208 SYNJ1_HUMAN (4-500): 4.14e-62: 222: 14.85		NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00
+	Hs
		166030 SYNJ2_HUMAN (3-471): 6.56e-60: 215: 14.85		NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00
+	Mm
		936473 WASP_MOUSE (16-487): 4.45e-46: 170: 39.21		NCK1_MOUSE (1-377): 7.48e-99: 300: 98.94
+	Rn
		192744 SYNJ1_RAT (4-505): 7.74e-69: 241: 13.31		SRBS2_RAT (3-162): 4.86e-25: 109: 28.05
+	Hs
		203192 SYNJ1_HUMAN (4-500): 4.14e-62: 222: 14.85		SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26
+	Hs
		166032 SYNJ2_HUMAN (3-471): 6.56e-60: 215: 14.85		P85A_HUMAN (241-376): 1.37e-22: 101: 19.57
+	Hs
		203230 SYNJ1_HUMAN (4-500): 4.14e-62: 222: 14.85		SH319_HUMAN (8-254): 6.26e-19: 90.5: 19.84
+	Hs
		166024 SYNJ2_HUMAN (3-471): 6.56e-60: 215: 14.85		FYN_HUMAN (7-173): 7.47e-21: 96: 15.39
+	Hs
		203228 SYNJ1_HUMAN (4-500): 4.14e-62: 222: 14.85		ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07
+	Hs
		203218 SYNJ1_HUMAN (4-500): 4.14e-62: 222: 14.85		GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81
+	Hs
		166049 SYNJ2_HUMAN (3-471): 6.56e-60: 215: 14.85		ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07
+	Hs
		166042 SYNJ2_HUMAN (3-471): 6.56e-60: 215: 14.85		GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81
+	Hs
		166056 SYNJ2_HUMAN (3-471): 6.56e-60: 215: 14.85		ITSN2_HUMAN (7-85): 2.68e-12: 70.6: 24.05
+	Hs
		203241 SYNJ1_HUMAN (4-500): 4.14e-62: 222: 14.85		SH3K1_HUMAN (58-207): 2.14e-09: 61.3: 17.76
+	Dm
		112580 Q9VAT0_DROME (1-504): 8.78e-57: 199: 31.37		M9ND00_DROME (1-66): 4.46e-15: 78.8: 26.47
+	Dm
		1361426 Q9VAT0_DROME (1-504): 8.78e-57: 199: 31.37		Q9VIF7_DROME (1-70): 4.36e-15: 78.8: 26.39
+	Hs
		166055 SYNJ2_HUMAN (3-471): 6.56e-60: 215: 14.85		SH3K1_HUMAN (58-207): 2.14e-09: 61.3: 17.76
+	Mm
		643073 WASP_MOUSE (16-487): 4.45e-46: 170: 39.21		P85A_MOUSE (241-376): 1.59e-22: 101: 19.57
+	Mm
		763384 WASP_MOUSE (16-487): 4.45e-46: 170: 39.21		FYN_MOUSE (7-173): 7.13e-21: 96: 15.39
+	Mm
		403427 WASP_MOUSE (16-487): 4.45e-46: 170: 39.21		SRC_MOUSE (5-114): 1.55e-15: 79.7: 22.32