HitPredict

Interactor 1: U2AF1 (Q94535)
Splicing factor U2af 38 kDa subunit (U2 auxiliary factor 38 kDa subunit) (U2 snRNP auxiliary factor small subunit)
Gene: U2af38

Interactor 2: Q9W1T4 (Q9W1T4)
Splicing factor U2AF subunit (U2 snRNP auxiliary factor large subunit)
Gene: LS2

Species: Drosophila melanogaster

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.775

Property	Details
Experiments	21406555, 14605208
Identification Methods	affinity chromatography technology, two hybrid
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	RRM_1~RRM_1, RRM_1~zf-CCCH
Common GO terms	GO:0003729 - mRNA binding GO:0008187 - poly-pyrimidine tract binding GO:0030628 - pre-mRNA 3'-splice site binding GO:0089701 - U2AF complex
Homologous interactions	10

Homologous interactions

Color coding for hit scores

	1 264 Dm			1 449
+	Sp
		737061 U2AF1_SCHPO (1-193): 2.31e-87: 260: 57.51		U2AF2_SCHPO (13-446): 2.22e-88: 280: 25.94
+	At
		188099 U2AFB_ARATH (1-228): 1.51e-85: 258: 48.75		U2A2A_ARATH (64-448): 6.42e-88: 280: 33.50
+	Dm
		1098215 U2AF1_DROME (1-251): 8.66e-105: 306: 100.00		U2AF2_DROME (54-448): 4.38e-67: 222: 53.52
+	Hs
		641723 U2AF1_HUMAN (1-188): 3.86e-100: 293: 82.98		U2AF2_HUMAN (37-448): 7.83e-65: 217: 45.92
+	Hs
		641801 U2AFL_HUMAN (12-250): 4.11e-93: 284: 32.93		U2AF2_HUMAN (37-448): 7.83e-65: 217: 45.92
+	Ce
		954186 Q9U2U0_CAEEL (2-203): 1.86e-90: 270: 62.56		U2AF2_CAEEL (40-448): 4.58e-69: 229: 43.52
+	Hs
		516470 U2AF1_HUMAN (1-188): 3.86e-100: 293: 82.98		PABP1_HUMAN (232-448): 8.67e-37: 144: 13.83
+	Hs
		963205 U2AF1_HUMAN (1-188): 3.86e-100: 293: 82.98		RBM39_HUMAN (107-444): 1.74e-31: 128: 18.32
+	At
		96446 U2AFA_ARATH (1-247): 2.4e-92: 276: 45.90		F4ILE1_ARATH (224-436): 2.35e-11: 67.6: 18.61
+	At
		1351706 U2AFA_ARATH (1-247): 2.4e-92: 276: 45.90		Q9ZVW9_ARATH (243-436): 8.68e-11: 66.1: 20.41