HitPredict

Interactor 1: UB2E3 (Q969T4)
Ubiquitin-conjugating enzyme E2 E3 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme E3) (UbcH9) (Ubiquitin carrier protein E3) (Ubiquitin-conjugating enzyme E2-23 kDa) (Ubiquitin-protein ligase E3)
Gene: UBE2E3

Interactor 2: RNF5 (Q99942)
E3 ubiquitin-protein ligase RNF5 (EC 2.3.2.27) (RING finger protein 5) (Ram1 homolog) (HsRma1)
Gene: RNF5

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	1.00	0.965

Property	Details
Experiments	32296183, 19549727, 25416956, 15545318, unassigned1304, 31515488, 14667819
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-C3HC4_3
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0070534 - protein K63-linked ubiquitination GO:0070936 - protein K48-linked ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 207 Hs			1 180
+	Mm
		192776 UB2E3_MOUSE (1-207): 5.71e-90: 264: 100.00		RNF5_MOUSE (1-164): 8.42e-56: 175: 97.56
+	Hs
		1297363 UB2E2_HUMAN (1-207): 5.59e-89: 261: 85.02		RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00
+	Hs
		1282328 UB2E3_HUMAN (1-207): 5.71e-90: 264: 100.00		RN185_HUMAN (17-179): 7.02e-52: 166: 60.98
+	Dm
		854578 UBCD2_DROME (2-207): 3.23e-82: 245: 71.36		Q9VXP1_DROME (3-178): 1.17e-57: 183: 47.73
+	Hs
		1292341 UB2E2_HUMAN (1-207): 5.59e-89: 261: 85.02		RN185_HUMAN (17-179): 7.02e-52: 166: 60.98
+	Hs
		1307272 UB2D4_HUMAN (61-206): 1.19e-82: 243: 63.01		RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00
+	Dm
		854562 UBCD2_DROME (2-207): 3.23e-82: 245: 71.36		Q8IR26_DROME (3-179): 2.28e-52: 170: 48.32
+	Hs
		1292368 UB2D4_HUMAN (61-206): 1.19e-82: 243: 63.01		RN185_HUMAN (17-179): 7.02e-52: 166: 60.98
+	Hs
		1282350 UB2E3_HUMAN (1-207): 5.71e-90: 264: 100.00		TRI39_HUMAN (24-148): 5.72e-26: 104: 21.43
+	Hs
		1297366 UB2E2_HUMAN (1-207): 5.59e-89: 261: 85.02		TRI31_HUMAN (19-126): 9.14e-27: 106: 20.91
+	Hs
		1297370 UB2E2_HUMAN (1-207): 5.59e-89: 261: 85.02		TRI39_HUMAN (24-148): 5.72e-26: 104: 21.43
+	Hs
		262192 UB2E2_HUMAN (1-207): 5.59e-89: 261: 85.02		TRIM3_HUMAN (3-109): 3.92e-21: 91.3: 19.84
+	Hs
		749752 UB2E3_HUMAN (1-207): 5.71e-90: 264: 100.00		BRCA1_HUMAN (25-76): 3.12e-19: 86.4: 34.62
+	Hs
		1318532 UB2D4_HUMAN (61-206): 1.19e-82: 243: 63.01		TRI31_HUMAN (19-126): 9.14e-27: 106: 20.91
+	Hs
		749782 UB2E2_HUMAN (1-207): 5.59e-89: 261: 85.02		BRCA1_HUMAN (25-76): 3.12e-19: 86.4: 34.62
+	Hs
		1336390 UB2D4_HUMAN (61-206): 1.19e-82: 243: 63.01		TRI39_HUMAN (24-148): 5.72e-26: 104: 21.43
+	Hs
		1354526 UB2D4_HUMAN (61-206): 1.19e-82: 243: 63.01		TRI10_HUMAN (14-156): 5.89e-24: 99: 17.45
+	Mm
		919925 UB2D2_MOUSE (61-206): 2.03e-82: 242: 65.07		TRI17_MOUSE (13-148): 1.18e-24: 100: 13.10
+	Hs
		262201 UB2D4_HUMAN (61-206): 1.19e-82: 243: 63.01		TRIM3_HUMAN (3-109): 3.92e-21: 91.3: 19.84
+	Hs
		262167 UB2D2_HUMAN (61-206): 2.03e-82: 242: 65.07		TRIM3_HUMAN (3-109): 3.92e-21: 91.3: 19.84