HitPredict

Interactor 1: UB2E3 (Q969T4)
Ubiquitin-conjugating enzyme E2 E3 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme E3) (UbcH9) (Ubiquitin carrier protein E3) (Ubiquitin-conjugating enzyme E2-23 kDa) (Ubiquitin-protein ligase E3)
Gene: UBE2E3

Interactor 2: HERC5 (Q9UII4)
E3 ISG15--protein ligase HERC5 (EC 2.3.2.-) (Cyclin-E-binding protein 1) (HECT domain and RCC1-like domain-containing protein 5)
Gene: HERC5

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.55	1.00	0.743

Property	Details
Experiments	35671810, 19549727
Identification Methods	affinity chromatography technology, two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	HECT~UQ_con
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 207 Hs			1 1024
+	Hs
		1297377 UB2E2_HUMAN (1-207): 5.59e-89: 261: 85.02		HERC5_HUMAN (1-1024): 0: 1123: 100.00
+	Hs
		125629 UB2E3_HUMAN (1-207): 5.71e-90: 264: 100.00		WWP2_HUMAN (599-1024): 1.14e-167: 516: 22.02
+	Hs
		125655 UB2E2_HUMAN (1-207): 5.59e-89: 261: 85.02		WWP2_HUMAN (599-1024): 1.14e-167: 516: 22.02
+	Hs
		1282329 UB2E3_HUMAN (1-207): 5.71e-90: 264: 100.00		ITCH_HUMAN (593-1024): 1.76e-165: 511: 24.22
+	Hs
		1282332 UB2E3_HUMAN (1-207): 5.71e-90: 264: 100.00		NED4L_HUMAN (652-1023): 2.49e-164: 510: 28.13
+	Hs
		1294835 UB2E2_HUMAN (1-207): 5.59e-89: 261: 85.02		ITCH_HUMAN (593-1024): 1.76e-165: 511: 24.22
+	Hs
		1297357 UB2E2_HUMAN (1-207): 5.59e-89: 261: 85.02		NED4L_HUMAN (652-1023): 2.49e-164: 510: 28.13
+	Hs
		919710 UB2D2_HUMAN (61-206): 2.03e-82: 242: 65.07		UBE3A_HUMAN (414-1024): 1.12e-172: 529: 25.37
+	Xl
		1151480 Q6WKX0_XENLA (1-207): 5.87e-89: 261: 99.03		Q6GMD5_XENLA (652-1023): 1.34e-163: 508: 27.34
+	Hs
		919860 UB2D2_HUMAN (61-206): 2.03e-82: 242: 65.07		WWP1_HUMAN (588-1024): 8.35e-169: 520: 21.93
+	Hs
		125808 UB2D4_HUMAN (61-206): 1.19e-82: 243: 63.01		WWP2_HUMAN (599-1024): 1.14e-167: 516: 22.02
+	Hs
		125335 UB2D2_HUMAN (61-206): 2.03e-82: 242: 65.07		WWP2_HUMAN (599-1024): 1.14e-167: 516: 22.02
+	Hs
		1294888 UB2D4_HUMAN (61-206): 1.19e-82: 243: 63.01		ITCH_HUMAN (593-1024): 1.76e-165: 511: 24.22
+	Hs
		808078 UB2D2_HUMAN (61-206): 2.03e-82: 242: 65.07		NEDD4_HUMAN (652-1023): 3.7e-161: 511: 26.04
+	Dm
		854579 UBCD2_DROME (2-207): 3.23e-82: 245: 71.36		SUDX_DROME (583-1023): 1.22e-163: 508: 21.08
+	Hs
		919827 UB2D2_HUMAN (61-206): 2.03e-82: 242: 65.07		ITCH_HUMAN (593-1024): 1.76e-165: 511: 24.22
+	Hs
		919834 UB2D2_HUMAN (61-206): 2.03e-82: 242: 65.07		NED4L_HUMAN (652-1023): 2.49e-164: 510: 28.13
+	Hs
		919762 UB2D2_HUMAN (61-206): 2.03e-82: 242: 65.07		HECW1_HUMAN (532-1024): 4.53e-156: 504: 22.94
+	Hs
		1125824 UB2E3_HUMAN (1-207): 5.71e-90: 264: 100.00		HERC4_HUMAN (317-431): 2.55e-13: 76.6: 11.02
+	Hs
		919741 UB2D2_HUMAN (61-206): 2.03e-82: 242: 65.07		HERC4_HUMAN (317-431): 2.55e-13: 76.6: 11.02