HitPredict

Interactor 1: UBE2W (Q96B02)
Ubiquitin-conjugating enzyme E2 W (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme W) (N-terminal E2 ubiquitin-conjugating enzyme) (EC 2.3.2.25) (N-terminus-conjugating E2) (Ubiquitin carrier protein W) (Ubiquitin-conjugating enzyme 16) (UBC-16) (Ubiquitin-protein ligase W)
Gene: UBE2W

Interactor 2: AMFR (Q9UKV5)
E3 ubiquitin-protein ligase AMFR (EC 2.3.2.36) (Autocrine motility factor receptor) (AMF receptor) (RING finger protein 45) (gp78)
Gene: AMFR

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.66	1.00	0.815

Property	Details
Experiments	19690564, 19549727
Identification Methods	two hybrid, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	G2BR~UQ_con, UQ_con~zf-RING_2
Common GO terms	GO:0000209 - protein polyubiquitination GO:0004842 - ubiquitin-protein transferase activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 151 Hs			1 643
+	Hs
		1356614 UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37		AMFR_HUMAN (1-643): 0: 593: 100.00
+	Hs
		849484 UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69		AMFR_HUMAN (1-643): 0: 593: 100.00
+	Hs
		230183 UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37		MGRN1_HUMAN (303-624): 2.06e-40: 157: 17.31
+	Hs
		230126 UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69		MGRN1_HUMAN (303-624): 2.06e-40: 157: 17.31
+	Hs
		1356934 UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37		RN150_HUMAN (258-488): 7.54e-36: 142: 15.26
+	Hs
		610305 UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69		CBL_HUMAN (293-550): 4.72e-35: 143: 19.37
+	Hs
		849485 UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69		RN150_HUMAN (258-488): 7.54e-36: 142: 15.26
+	Hs
		262201 UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37		TRIM3_HUMAN (327-603): 4.69e-32: 134: 13.81
+	Hs
		262161 UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69		TRIM3_HUMAN (327-603): 4.69e-32: 134: 13.81
+	Hs
		1304199 UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37		RING2_HUMAN (315-579): 7.78e-32: 128: 14.44
+	Zm
		74334 B4FC97_MAIZE (4-150): 5.6e-69: 206: 32.65		B6U926_MAIZE (245-437): 2.42e-32: 130: 15.14
+	Zm
		79637 B6SZE0_MAIZE (4-150): 5.92e-69: 206: 31.97		B6U926_MAIZE (245-437): 2.42e-32: 130: 15.14
+	Sc
		549855 UBC5_YEAST (1-150): 2.23e-68: 205: 32.67		TUL1_YEAST (156-384): 9.9e-31: 130: 14.07
+	Hs
		849421 UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69		RING2_HUMAN (315-579): 7.78e-32: 128: 14.44
+	Hs
		1377467 UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37		RN115_HUMAN (316-401): 6.66e-25: 107: 26.67
+	Hs
		849497 UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69		RN115_HUMAN (316-401): 6.66e-25: 107: 26.67
+	Hs
		849449 UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69		RN122_HUMAN (269-391): 1.51e-24: 101: 23.02
+	Hs
		849382 UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69		RN128_HUMAN (260-411): 9.48e-22: 100: 15.34
+	Hs
		1205159 UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37		DZIP3_HUMAN (341-387): 1.42e-10: 66.7: 38.78
+	Hs
		849362 UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69		DZIP3_HUMAN (341-387): 1.42e-10: 66.7: 38.78