HitPredict

Interactor 1: CHFR (Q96EP1)
E3 ubiquitin-protein ligase CHFR (EC 2.3.2.27) (Checkpoint with forkhead and RING finger domains protein) (RING finger protein 196) (RING-type E3 ubiquitin transferase CHFR)
Gene: CHFR

Interactor 2: UB2E2 (Q96LR5)
Ubiquitin-conjugating enzyme E2 E2 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme E2) (UbcH8) (Ubiquitin carrier protein E2) (Ubiquitin-protein ligase E2)
Gene: UBE2E2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	32296183, 19549727, unassigned1304
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-C3HC4_2
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005634 - nucleus
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 664 Hs			1 201
+	Hs
		1282325 CHFR_HUMAN (1-664): 0: 904: 100.00		UB2E3_HUMAN (1-201): 6.37e-85: 251: 79.71
+	Hs
		1290127 CHFR_HUMAN (1-664): 0: 904: 100.00		UB2D4_HUMAN (55-200): 9.37e-83: 243: 63.01
+	Hs
		919820 CHFR_HUMAN (1-664): 0: 904: 100.00		UB2D2_HUMAN (55-200): 2.8e-82: 242: 64.38
+	Hs
		849404 CHFR_HUMAN (1-664): 0: 904: 100.00		UB2D1_HUMAN (55-200): 1.23e-81: 240: 61.64
+	Hs
		851866 CHFR_HUMAN (1-664): 0: 904: 100.00		UB2E1_HUMAN (28-201): 6.5e-80: 238: 89.71
+	Hs
		895874 CHFR_HUMAN (1-664): 0: 904: 100.00		UB2D3_HUMAN (55-200): 1.28e-80: 238: 63.70
+	Hs
		896515 CHFR_HUMAN (1-664): 0: 904: 100.00		UBE2N_HUMAN (53-200): 2.86e-70: 212: 45.27
+	Mm
		896587 CHFR_MOUSE (16-664): 0: 877: 82.98		UBE2N_MOUSE (53-200): 2.86e-70: 212: 45.27
+	Hs
		282716 RNF8_HUMAN (37-385): 7.97e-108: 336: 19.15		UB2E2_HUMAN (1-201): 6.02e-94: 274: 100.00
+	Hs
		282705 RNF8_HUMAN (37-385): 7.97e-108: 336: 19.15		UB2E3_HUMAN (1-201): 6.37e-85: 251: 79.71
+	Hs
		282607 RNF8_HUMAN (37-385): 7.97e-108: 336: 19.15		UB2D2_HUMAN (55-200): 2.8e-82: 242: 64.38
+	Hs
		282581 RNF8_HUMAN (37-385): 7.97e-108: 336: 19.15		UB2D1_HUMAN (55-200): 1.23e-81: 240: 61.64
+	Hs
		282582 RNF8_HUMAN (37-385): 7.97e-108: 336: 19.15		UB2E1_HUMAN (28-201): 6.5e-80: 238: 89.71
+	Hs
		282595 RNF8_HUMAN (37-385): 7.97e-108: 336: 19.15		UB2D3_HUMAN (55-200): 1.28e-80: 238: 63.70
+	Hs
		282596 RNF8_HUMAN (37-385): 7.97e-108: 336: 19.15		UBE2N_HUMAN (53-200): 2.86e-70: 212: 45.27
+	Mm
		896589 RNF8_MOUSE (37-385): 1.38e-102: 323: 18.68		UBE2N_MOUSE (53-200): 2.86e-70: 212: 45.27
+	Hs
		1108530 PCGF3_HUMAN (290-431): 4.18e-40: 148: 20.13		UB2E3_HUMAN (1-201): 6.37e-85: 251: 79.71
+	Zm
		13425 A0A1D6FSN7_MAIZE (188-499): 1.01e-38: 151: 12.66		B4FC97_MAIZE (55-200): 8.66e-82: 241: 62.33
+	Hs
		717615 BMI1_HUMAN (287-438): 5.06e-39: 148: 19.62		UB2D3_HUMAN (55-200): 1.28e-80: 238: 63.70
+	Hs
		895802 PCGF3_HUMAN (290-431): 4.18e-40: 148: 20.13		UB2D3_HUMAN (55-200): 1.28e-80: 238: 63.70