HitPredict

Interactor 1: RN185 (Q96GF1)
E3 ubiquitin-protein ligase RNF185 (EC 2.3.2.27) (RING finger protein 185)
Gene: RNF185

Interactor 2: UB2J1 (Q9Y385)
Ubiquitin-conjugating enzyme E2 J1 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme J1) (Non-canonical ubiquitin-conjugating enzyme 1) (NCUBE-1) (Yeast ubiquitin-conjugating enzyme UBC6 homolog E) (HsUBC6e)
Gene: UBE2J1

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.38	1.00	0.618

Property	Details
Experiments	24019521
Identification Methods	affinity chromatography technology
Interaction Type	physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-C3HC4
Common GO terms	GO:0005789 - endoplasmic reticulum membrane GO:0036503 - ERAD pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 192 Hs			1 318
+	Hs
		1292368 RN185_HUMAN (1-192): 2.35e-56: 178: 100.00		UB2D4_HUMAN (10-156): 3.97e-70: 216: 28.00
+	Hs
		919826 RN185_HUMAN (1-192): 2.35e-56: 178: 100.00		UB2D2_HUMAN (10-156): 5.15e-70: 216: 28.00
+	Hs
		849409 RN185_HUMAN (1-192): 2.35e-56: 178: 100.00		UB2D1_HUMAN (10-156): 1e-69: 215: 30.00
+	Hs
		895880 RN185_HUMAN (1-192): 2.35e-56: 178: 100.00		UB2D3_HUMAN (10-156): 1.73e-68: 212: 28.00
+	Dm
		639411 Q9VXP1_DROME (15-192): 4.58e-51: 167: 58.43		UBCD1_DROME (10-156): 3.06e-69: 214: 29.33
+	Hs
		851870 RN185_HUMAN (1-192): 2.35e-56: 178: 100.00		UB2E1_HUMAN (1-156): 1.67e-64: 203: 28.30
+	Hs
		1231675 RN185_HUMAN (1-192): 2.35e-56: 178: 100.00		UB2J2_HUMAN (1-150): 3.16e-63: 202: 39.87
+	Hs
		1282328 RN185_HUMAN (1-192): 2.35e-56: 178: 100.00		UB2E3_HUMAN (2-156): 9.16e-63: 199: 26.58
+	Hs
		1292341 RN185_HUMAN (1-192): 2.35e-56: 178: 100.00		UB2E2_HUMAN (2-156): 2.19e-62: 198: 27.22
+	Dm
		639398 Q8IR26_DROME (15-191): 8.57e-45: 151: 52.54		UBCD1_DROME (10-156): 3.06e-69: 214: 29.33
+	Dm
		854578 Q9VXP1_DROME (15-192): 4.58e-51: 167: 58.43		UBCD2_DROME (5-156): 8.48e-61: 195: 27.74
+	Dm
		854562 Q8IR26_DROME (15-191): 8.57e-45: 151: 52.54		UBCD2_DROME (5-156): 8.48e-61: 195: 27.74
+	Hs
		919838 RNF5_HUMAN (15-173): 2.18e-37: 129: 60.38		UB2D2_HUMAN (10-156): 5.15e-70: 216: 28.00
+	Hs
		849424 RNF5_HUMAN (15-173): 2.18e-37: 129: 60.38		UB2D1_HUMAN (10-156): 1e-69: 215: 30.00
+	Hs
		895892 RNF5_HUMAN (15-173): 2.18e-37: 129: 60.38		UB2D3_HUMAN (10-156): 1.73e-68: 212: 28.00
+	Hs
		851879 RNF5_HUMAN (15-173): 2.18e-37: 129: 60.38		UB2E1_HUMAN (1-156): 1.67e-64: 203: 28.30
+	Hs
		1282336 RNF5_HUMAN (15-173): 2.18e-37: 129: 60.38		UB2E3_HUMAN (2-156): 9.16e-63: 199: 26.58
+	Mm
		192776 RNF5_MOUSE (15-173): 2.46e-37: 129: 61.01		UB2E3_MOUSE (2-156): 9.16e-63: 199: 26.58
+	Hs
		1297363 RNF5_HUMAN (15-173): 2.18e-37: 129: 60.38		UB2E2_HUMAN (2-156): 2.19e-62: 198: 27.22
+	Hs
		891430 RNF5_HUMAN (15-173): 2.18e-37: 129: 60.38		UB2G2_HUMAN (7-142): 5.84e-58: 185: 26.00