HitPredict

Interactor 1: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Interactor 2: SH3G3 (Q99963)
Endophilin-A3 (EEN-B2) (Endophilin-3) (SH3 domain protein 2C) (SH3 domain-containing GRB2-like protein 3)
Gene: SH3GL3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.54	0.60	0.571

Property	Details
Experiments	31980649, unassigned2173
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0031901 - early endosome membrane
Homologous interactions	11

Homologous interactions

Color coding for hit scores

	1 903 Hs			1 347
+	Hs
		1294845 ITCH_HUMAN (1-903): 0: 926: 100.00		SH3G2_HUMAN (1-347): 8.3e-86: 264: 65.91
+	Hs
		1294844 ITCH_HUMAN (1-903): 0: 926: 100.00		SH3G1_HUMAN (1-347): 7.8e-80: 249: 66.30
+	Hs
		1294889 ITCH_HUMAN (1-903): 0: 926: 100.00		SHLB1_HUMAN (10-342): 1.46e-66: 215: 24.22
+	Hs
		1294851 ITCH_HUMAN (1-903): 0: 926: 100.00		PACN1_HUMAN (6-342): 5.44e-34: 131: 14.90
+	Hs
		1078893 ITCH_HUMAN (1-903): 0: 926: 100.00		ITSN1_HUMAN (253-341): 7.42e-12: 68.9: 23.60
+	Hs
		125453 WWP2_HUMAN (1-903): 0: 816: 54.08		SRC8_HUMAN (7-343): 2.64e-50: 177: 14.49
+	Hs
		125348 WWP2_HUMAN (1-903): 0: 816: 54.08		GRB2_HUMAN (290-346): 9.93e-12: 65.5: 41.38
+	Hs
		125507 WWP2_HUMAN (1-903): 0: 816: 54.08		SH319_HUMAN (291-342): 6.81e-09: 59.3: 48.08
+	Hs
		1052323 SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71		SRC8_HUMAN (7-343): 2.64e-50: 177: 14.49
+	Hs
		596243 SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94		NEBU_HUMAN (7-342): 2.57e-37: 144: 16.06
+	Hs
		1350514 HECW2_HUMAN (1-512): 1.38e-13: 77.2: 11.34		3BP1_HUMAN (6-252): 2.26e-38: 146: 17.63