HitPredict

Interactor 1: HTF4 (Q99081)
Transcription factor 12 (TCF-12) (Class B basic helix-loop-helix protein 20) (bHLHb20) (DNA-binding protein HTF4) (E-box-binding protein) (Transcription factor HTF-4)
Gene: TCF12

Interactor 2: ASCL3 (Q9NQ33)
Achaete-scute homolog 3 (ASH-3) (hASH3) (Class A basic helix-loop-helix protein 42) (bHLHa42) (bHLH transcriptional regulator Sgn-1)
Gene: ASCL3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.73	1.00	0.858

Property	Details
Experiments	32296183, 33961781, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, validated two hybrid, two hybrid array, two hybrid prey pooling approach, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	HLH~HLH
Common GO terms	GO:0000785 - chromatin GO:0000981 - DNA-binding transcription factor activity, RNA polymerase II-specific GO:0006357 - regulation of transcription by RNA polymerase II GO:0045944 - positive regulation of transcription by RNA polymerase II GO:0090575 - RNA polymerase II transcription regulator complex
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 682 Hs			1 181
+	Hs
		551571 HTF4_HUMAN (1-682): 0: 921: 100.00		TFE2_HUMAN (5-178): 2.08e-28: 112: 17.42
+	Hs
		899129 HTF4_HUMAN (1-682): 0: 921: 100.00		HAND2_HUMAN (35-163): 8.1e-30: 110: 21.37
+	Hs
		616615 HTF4_HUMAN (1-682): 0: 921: 100.00		MYF6_HUMAN (29-157): 5.89e-29: 109: 27.14
+	Hs
		1303321 HTF4_HUMAN (1-682): 0: 921: 100.00		NDF4_HUMAN (85-181): 1.01e-27: 107: 26.80
+	Hs
		568226 HTF4_HUMAN (1-682): 0: 921: 100.00		TAL1_HUMAN (53-158): 3.16e-27: 106: 35.35
+	Hs
		1018388 HTF4_HUMAN (1-682): 0: 921: 100.00		TCF15_HUMAN (29-175): 1.3e-27: 104: 22.22
+	Hs
		1039378 HTF4_HUMAN (1-682): 0: 921: 100.00		NDF1_HUMAN (85-181): 3.01e-26: 104: 27.84
+	Hs
		1271845 HTF4_HUMAN (1-682): 0: 921: 100.00		ATOH1_HUMAN (75-179): 2.08e-26: 104: 24.76
+	Hs
		1303333 HTF4_HUMAN (1-682): 0: 921: 100.00		NGN3_HUMAN (23-176): 1.56e-27: 104: 20.96
+	Hs
		1167653 HTF4_HUMAN (1-682): 0: 921: 100.00		ASCL4_HUMAN (14-172): 1.54e-27: 103: 37.11
+	Hs
		1272169 HTF4_HUMAN (1-682): 0: 921: 100.00		NGN1_HUMAN (3-176): 1.7e-26: 102: 20.23
+	Hs
		210863 HTF4_HUMAN (1-682): 0: 921: 100.00		TCF21_HUMAN (78-157): 1.17e-26: 101: 28.40
+	Hs
		330430 HTF4_HUMAN (1-682): 0: 921: 100.00		HAND1_HUMAN (25-180): 4.15e-26: 101: 20.76
+	Hs
		1300900 HTF4_HUMAN (1-682): 0: 921: 100.00		FER3L_HUMAN (82-158): 4.16e-26: 99.5: 35.07
+	Hs
		971805 HTF4_HUMAN (1-682): 0: 921: 100.00		HEN1_HUMAN (93-150): 1.85e-24: 94.5: 43.10
+	Hs
		1261463 HTF4_HUMAN (1-682): 0: 921: 100.00		TWST2_HUMAN (35-157): 5.32e-24: 94.1: 23.58
+	Hs
		1083176 HTF4_HUMAN (1-682): 0: 921: 100.00		TAL2_HUMAN (99-156): 3.51e-24: 92.9: 43.10
+	Mm
		603985 HTF4_MOUSE (1-682): 0: 907: 92.21		TAL1_MOUSE (53-162): 1.43e-26: 104: 35.00
+	Hs
		238594 HTF4_HUMAN (1-682): 0: 921: 100.00		MUSC_HUMAN (83-157): 1.2e-12: 65.2: 30.67
+	Mm
		644476 HTF4_MOUSE (1-682): 0: 907: 92.21		TWST1_MOUSE (98-157): 3.28e-11: 61.3: 41.67