HitPredict

Interactor 1: EPAS1 (Q99814)
Endothelial PAS domain-containing protein 1 (EPAS-1) (Basic-helix-loop-helix-PAS protein MOP2) (Class E basic helix-loop-helix protein 73) (bHLHe73) (HIF-1-alpha-like factor) (HLF) (Hypoxia-inducible factor 2-alpha) (HIF-2-alpha) (HIF2-alpha) (Member of PAS protein 2) (PAS domain-containing protein 2)
Gene: EPAS1

Interactor 2: EGLN3 (Q9H6Z9)
Prolyl hydroxylase EGLN3 (EC 1.14.11.-) (Egl nine homolog 3) (EC 1.14.11.29) (HPH-1) (Hypoxia-inducible factor prolyl hydroxylase 3) (HIF-PH3) (HIF-prolyl hydroxylase 3) (HPH-3) (Prolyl hydroxylase domain-containing protein 3) (PHD3)
Gene: EGLN3

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.71	1.00	0.844

Property	Details
Experiments	17684156, 17353276, 23275444
Identification Methods	two hybrid, pull down, affinity chromatography technology
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	2OG-FeII_Oxy_3~HIF-1
Common GO terms	GO:0001666 - response to hypoxia GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0071456 - cellular response to hypoxia
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 870 Hs			1 239
+	Hs
		1306535 EPAS1_HUMAN (1-870): 0: 863: 100.00		EGLN1_HUMAN (2-225): 3.05e-143: 409: 64.29
+	Hs
		1296543 EPAS1_HUMAN (1-870): 0: 863: 100.00		EGLN2_HUMAN (12-226): 6.89e-132: 379: 61.86
+	Hs
		1085680 HIF1A_HUMAN (3-868): 0: 553: 42.32		EGLN3_HUMAN (1-239): 5.66e-162: 449: 100.00
+	Hs
		87731 D0VY79_HUMAN (3-868): 0: 553: 42.32		EGLN3_HUMAN (1-239): 5.66e-162: 449: 100.00
+	Mm
		1142404 HIF1A_MOUSE (3-787): 0: 549: 43.60		EGLN3_MOUSE (1-239): 5.54e-161: 446: 97.07
+	Hs
		1085668 HIF1A_HUMAN (3-868): 0: 553: 42.32		EGLN1_HUMAN (2-225): 3.05e-143: 409: 64.29
+	Mm
		1142406 HIF1A_MOUSE (3-787): 0: 549: 43.60		EGLN1_MOUSE (2-225): 7.91e-145: 412: 64.73
+	Mm
		1142405 HIF1A_MOUSE (3-787): 0: 549: 43.60		EGLN2_MOUSE (5-226): 5.91e-134: 385: 60.36
+	Hs
		1085645 HIF1A_HUMAN (3-868): 0: 553: 42.32		EGLN2_HUMAN (12-226): 6.89e-132: 379: 61.86
+	Hs
		649159 ARNT_HUMAN (2-644): 5.31e-129: 407: 19.24		EGLN3_HUMAN (1-239): 5.66e-162: 449: 100.00
+	Hs
		728960 AHR_HUMAN (5-706): 9.13e-121: 387: 16.46		EGLN3_HUMAN (1-239): 5.66e-162: 449: 100.00
+	Hs
		649156 ARNT_HUMAN (2-644): 5.31e-129: 407: 19.24		EGLN1_HUMAN (2-225): 3.05e-143: 409: 64.29
+	Hs
		649144 ARNT_HUMAN (2-644): 5.31e-129: 407: 19.24		EGLN2_HUMAN (12-226): 6.89e-132: 379: 61.86
+	Hs
		1075392 NCOA2_HUMAN (6-564): 4.76e-97: 334: 18.53		EGLN3_HUMAN (1-239): 5.66e-162: 449: 100.00