HitPredict

Interactor 1: RNF5 (Q99942)
E3 ubiquitin-protein ligase RNF5 (EC 2.3.2.27) (RING finger protein 5) (Ram1 homolog) (HsRma1)
Gene: RNF5

Interactor 2: UB2D4 (Q9Y2X8)
Ubiquitin-conjugating enzyme E2 D4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D4) (HBUCE1) (Ubiquitin carrier protein D4) (Ubiquitin-protein ligase D4)
Gene: UBE2D4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.82	1.00	0.911

Property	Details
Experiments	25416956, 32296183, 19549727, unassigned1304
Identification Methods	two hybrid, validated two hybrid, two hybrid prey pooling approach, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-C3HC4_3
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0006511 - ubiquitin-dependent protein catabolic process GO:0070534 - protein K63-linked ubiquitination GO:0070936 - protein K48-linked ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 180 Hs			1 147
+	Dm
		639411 Q9VXP1_DROME (3-178): 1.17e-57: 183: 47.73		UBCD1_DROME (1-147): 4.66e-82: 239: 92.52
+	Hs
		849424 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		919838 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		895892 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Dm
		639398 Q8IR26_DROME (3-179): 2.28e-52: 170: 48.32		UBCD1_DROME (1-147): 4.66e-82: 239: 92.52
+	Hs
		849409 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Dm
		854578 Q9VXP1_DROME (3-178): 1.17e-57: 183: 47.73		UBCD2_DROME (4-146): 7.36e-75: 224: 65.73
+	Hs
		919826 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		895880 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		1297363 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2E2_HUMAN (4-146): 3.5e-75: 224: 64.34
+	Hs
		1282336 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Mm
		192776 RNF5_MOUSE (1-164): 8.42e-56: 175: 97.56		UB2E3_MOUSE (4-146): 1.24e-74: 223: 64.34
+	Dm
		854562 Q8IR26_DROME (3-179): 2.28e-52: 170: 48.32		UBCD2_DROME (4-146): 7.36e-75: 224: 65.73
+	Hs
		851879 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Hs
		1292341 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2E2_HUMAN (4-146): 3.5e-75: 224: 64.34
+	Hs
		1282328 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Hs
		851870 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Hs
		896528 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Dm
		1337333 Q9VXP1_DROME (3-178): 1.17e-57: 183: 47.73		Q9I7T6_DROME (1-146): 3.53e-50: 165: 52.74
+	Dm
		1215059 Q8IR26_DROME (3-179): 2.28e-52: 170: 48.32		Q9I7T6_DROME (1-146): 3.53e-50: 165: 52.74