HitPredict

Interactor 1: MLP3C (Q9BXW4)
Microtubule-associated proteins 1A/1B light chain 3C (Autophagy-related protein LC3 C) (Autophagy-related ubiquitin-like modifier LC3 C) (MAP1 light chain 3-like protein 3) (MAP1A/MAP1B light chain 3 C) (MAP1A/MAP1B LC3 C) (Microtubule-associated protein 1 light chain 3 gamma)
Gene: MAP1LC3C

Interactor 2: ATG4B (Q9Y4P1)
Cysteine protease ATG4B (EC 3.4.22.-) (AUT-like 1 cysteine endopeptidase) (Autophagy-related cysteine endopeptidase 1) (Autophagin-1) (Autophagy-related protein 4 homolog B) (HsAPG4B) (hAPG4B)
Gene: ATG4B

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.86	1.00	0.928

Property	Details
Experiments	32296183, 28287329, 31857374, unassigned1304, 20562859
Identification Methods	two hybrid, pull down, affinity chromatography technology, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	ATG8~Peptidase_C54
Common GO terms	GO:0000045 - autophagosome assembly GO:0000421 - autophagosome membrane GO:0005829 - cytosol GO:0016236 - macroautophagy GO:0031410 - cytoplasmic vesicle and 1 others more... GO:0035973 - aggrephagy
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 147 Hs			1 393
+	Hs
		306368 GBRAP_HUMAN (13-127): 3.44e-54: 168: 38.26		ATG4B_HUMAN (1-393): 0: 634: 100.00
+	Hs
		891370 GBRL2_HUMAN (13-126): 1.65e-54: 168: 42.98		ATG4B_HUMAN (1-393): 0: 634: 100.00
+	Hs
		1330686 MLP3A_HUMAN (7-126): 2.49e-53: 165: 59.17		ATG4B_HUMAN (1-393): 0: 634: 100.00
+	Hs
		1326231 MLP3B_HUMAN (7-127): 8.15e-53: 164: 54.55		ATG4B_HUMAN (1-393): 0: 634: 100.00
+	Mm
		1206409 MLP3A_MOUSE (7-126): 2.49e-53: 165: 59.17		ATG4B_MOUSE (1-393): 0: 630: 94.91
+	Hs
		306253 GBRAP_HUMAN (13-127): 3.44e-54: 168: 38.26		ATG4A_HUMAN (10-368): 2.51e-171: 485: 55.53
+	Hs
		891248 GBRL2_HUMAN (13-126): 1.65e-54: 168: 42.98		ATG4A_HUMAN (10-368): 2.51e-171: 485: 55.53
+	Hs
		1264550 MLP3A_HUMAN (7-126): 2.49e-53: 165: 59.17		ATG4A_HUMAN (10-368): 2.51e-171: 485: 55.53
+	Hs
		1264548 MLP3B_HUMAN (7-127): 8.15e-53: 164: 54.55		ATG4A_HUMAN (10-368): 2.51e-171: 485: 55.53
+	Hs
		1264549 GBRL1_HUMAN (13-126): 4.75e-52: 162: 39.47		ATG4A_HUMAN (10-368): 2.51e-171: 485: 55.53
+	Ce
		111920 LGG1_CAEEL (12-126): 1.46e-52: 164: 38.26		ATG41_CAEEL (5-393): 7.98e-166: 475: 37.64
+	Hs
		306219 GBRAP_HUMAN (13-127): 3.44e-54: 168: 38.26		ATG4D_HUMAN (24-391): 4.43e-155: 447: 30.58
+	Hs
		891203 GBRL2_HUMAN (13-126): 1.65e-54: 168: 42.98		ATG4D_HUMAN (24-391): 4.43e-155: 447: 30.58
+	Hs
		1198602 MLP3B_HUMAN (7-127): 8.15e-53: 164: 54.55		ATG4D_HUMAN (24-391): 4.43e-155: 447: 30.58
+	Hs
		1198603 GBRL1_HUMAN (13-126): 4.75e-52: 162: 39.47		ATG4D_HUMAN (24-391): 4.43e-155: 447: 30.58
+	Dm
		1372164 Q9W2S2_DROME (13-127): 6.14e-55: 170: 37.39		Q9VPW2_DROME (14-358): 5.47e-133: 388: 44.13
+	Hs
		306269 GBRAP_HUMAN (13-127): 3.44e-54: 168: 38.26		ATG4C_HUMAN (22-391): 2.09e-127: 376: 24.94
+	Hs
		1288558 MLP3B_HUMAN (7-127): 8.15e-53: 164: 54.55		ATG4C_HUMAN (22-391): 2.09e-127: 376: 24.94
+	Ce
		999365 LGG1_CAEEL (12-126): 1.46e-52: 164: 38.26		ATG42_CAEEL (25-355): 2.36e-108: 329: 29.47
+	Sc
		746022 ATG8_YEAST (11-126): 1.29e-54: 169: 38.79		ATG4_YEAST (28-342): 6.14e-93: 289: 27.75