HitPredict

Interactor 1: RHOJ (Q9H4E5)
Rho-related GTP-binding protein RhoJ (Ras-like protein family member 7B) (Tc10-like GTP-binding protein)
Gene: RHOJ

Interactor 2: PAK6 (Q9NQU5)
Serine/threonine-protein kinase PAK 6 (EC 2.7.11.1) (PAK-5) (p21-activated kinase 6) (PAK-6)
Gene: PAK6

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.65	0.80	0.725

Property	Details
Experiments	25416956, 31515488
Identification Methods	two hybrid, validated two hybrid, two hybrid prey pooling approach, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	PBD~Ras, Pkinase~Ras
Common GO terms	-
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 214 Hs			1 681
+	Hs
		894474 CDC42_HUMAN (20-204): 2.24e-92: 270: 63.78		PAK6_HUMAN (1-681): 0: 641: 100.00
+	Hs
		894540 CDC42_HUMAN (20-204): 2.24e-92: 270: 63.78		PAK5_HUMAN (1-676): 1.65e-174: 516: 44.51
+	Hs
		1330966 RHOJ_HUMAN (1-214): 2.19e-84: 250: 100.00		PAK5_HUMAN (1-676): 1.65e-174: 516: 44.51
+	Hs
		330933 CDC42_HUMAN (20-204): 2.24e-92: 270: 63.78		PAK4_HUMAN (1-675): 1.1e-156: 466: 43.79
+	Hs
		331085 RHOJ_HUMAN (1-214): 2.19e-84: 250: 100.00		PAK4_HUMAN (1-675): 1.1e-156: 466: 43.79
+	Hs
		568605 CDC42_HUMAN (20-204): 2.24e-92: 270: 63.78		KAPCA_HUMAN (384-680): 1.08e-129: 388: 24.44
+	Hs
		568794 RHOJ_HUMAN (1-214): 2.19e-84: 250: 100.00		KAPCA_HUMAN (384-680): 1.08e-129: 388: 24.44
+	Ce
		983033 CDC42_CAEEL (20-205): 6.19e-84: 248: 63.30		PK1_CAEEL (161-680): 9.16e-127: 389: 33.58
+	Dm
		776940 CDC42_DROME (20-204): 9.91e-84: 248: 63.64		Q9VEV1_DROME (356-676): 5.65e-122: 376: 40.87
+	Dd
		710122 RACC_DICDI (20-204): 4.72e-92: 269: 55.68		PAKB_DICDI (388-676): 6.93e-65: 231: 45.39
+	Mm
		103565 CDC42_MOUSE (20-204): 2.24e-92: 270: 63.78		G5E884_MOUSE (3-205): 2.63e-39: 154: 17.16
+	Rn
		723755 CDC42_RAT (20-204): 2.24e-92: 270: 63.78		PAK1_RAT (3-205): 2.6e-39: 154: 17.16
+	Mm
		292039 CDC42_MOUSE (20-204): 2.24e-92: 270: 63.78		PAK1_MOUSE (3-205): 7.18e-39: 153: 16.67
+	Hs
		893957 CDC42_HUMAN (20-204): 2.24e-92: 270: 63.78		PAK1_HUMAN (3-205): 4.77e-39: 153: 17.16
+	Hs
		277599 CDC42_HUMAN (20-204): 2.24e-92: 270: 63.78		PAK3_HUMAN (3-215): 8.38e-34: 138: 14.78
+	Rn
		1145152 CDC42_RAT (20-204): 2.24e-92: 270: 63.78		PAK2_RAT (9-162): 1.11e-33: 137: 19.26
+	Hs
		893958 CDC42_HUMAN (20-204): 2.24e-92: 270: 63.78		PAK2_HUMAN (9-162): 1.11e-33: 137: 18.63
+	Hs
		1026226 RHOJ_HUMAN (1-214): 2.19e-84: 250: 100.00		PAK1_HUMAN (3-205): 4.77e-39: 153: 17.16
+	Hs
		277619 RHOJ_HUMAN (1-214): 2.19e-84: 250: 100.00		PAK3_HUMAN (3-215): 8.38e-34: 138: 14.78
+	Hs
		1026945 RHOJ_HUMAN (1-214): 2.19e-84: 250: 100.00		PAK2_HUMAN (9-162): 1.11e-33: 137: 18.63