HitPredict

Interactor 1: DRB1 (O04492)
Double-stranded RNA-binding protein 1 (Protein HYPONASTIC LEAVES 1) (dsRNA-binding protein 1) (AtDRB1)
Gene: DRB1

Interactor 2: DCL1 (Q9SP32)
Endoribonuclease Dicer homolog 1 (EC 3.1.26.-) (Dicer-like protein 1) (AtDCL1) (Protein ABNORMAL SUSPENSOR 1) (Protein CARPEL FACTORY) (Protein SHORT INTEGUMENTS 1) (Protein SUSPENSOR 1)
Gene: DCL1

Species: Arabidopsis thaliana

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.90	1.00	0.954

Property	Details
Experiments	25294831, 15821876, 17442570, 16428603, 20106953, 23886622
Identification Methods	two hybrid, pull down, far western blotting, protein complementation assay, affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	dsrm~dsrm, Helicase_C~dsrm, Ribonuclease_3~dsrm
Common GO terms	GO:0003725 - double-stranded RNA binding GO:0004525 - ribonuclease III activity GO:0005634 - nucleus GO:0031054 - pre-miRNA processing
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 419 At			1 1909
+	At
		1352640 DCL1_ARATH (2-171): 2.29e-30: 126: 16.92		DCL1_ARATH (1-1909): 0: 1623: 100.00
+	At
		138410 DRB1_ARATH (1-419): 1.99e-169: 482: 100.00		DCL3_ARATH (213-1906): 0: 879: 27.67
+	At
		138399 DRB1_ARATH (1-419): 1.99e-169: 482: 100.00		DCL2_ARATH (250-1796): 0: 852: 29.30
+	At
		952787 DRB4_ARATH (14-262): 2.63e-53: 183: 28.11		DCL4_ARATH (237-1907): 0: 993: 27.89
+	At
		1108230 DCL1_ARATH (2-171): 2.29e-30: 126: 16.92		DCL2_ARATH (250-1796): 0: 852: 29.30
+	Hs
		269593 PRKRA_HUMAN (3-201): 6.06e-54: 183: 18.27		RIGI_HUMAN (224-818): 1.07e-111: 381: 22.37
+	Hs
		269869 PRKRA_HUMAN (3-201): 6.06e-54: 183: 18.27		IFIH1_HUMAN (238-811): 1.45e-107: 371: 22.19
+	Hs
		321872 E2AK2_HUMAN (7-218): 1.31e-43: 162: 19.73		RIGI_HUMAN (224-818): 1.07e-111: 381: 22.37
+	Dm
		55497 Q9VLW8_DROME (13-293): 3.32e-56: 189: 17.18		DCR2_DROME (248-1225): 1.56e-93: 339: 18.80
+	Hs
		322980 STAU1_HUMAN (13-225): 7.67e-47: 171: 17.72		DHX58_HUMAN (247-801): 4.36e-100: 340: 22.63
+	Hs
		585524 E2AK2_HUMAN (7-218): 1.31e-43: 162: 19.73		DHX58_HUMAN (247-801): 4.36e-100: 340: 22.63
+	Hs
		133809 STAU1_HUMAN (13-225): 7.67e-47: 171: 17.72		DDX3X_HUMAN (54-488): 3.45e-78: 275: 13.54
+	Hs
		322157 STAU1_HUMAN (13-225): 7.67e-47: 171: 17.72		DDX5_HUMAN (128-510): 4.34e-64: 232: 10.69
+	Hs
		322951 STAU1_HUMAN (13-225): 7.67e-47: 171: 17.72		DDX17_HUMAN (169-481): 5.57e-62: 229: 12.88
+	Dm
		1363205 LOQS_DROME (12-194): 3.67e-43: 158: 16.51		DCR1_DROME (247-981): 4.09e-29: 130: 12.42
+	Hs
		269939 PRKRA_HUMAN (3-201): 6.06e-54: 183: 18.27		DICER_HUMAN (1565-1685): 7.57e-12: 73.1: 25.20
+	Hs
		877372 DSRAD_HUMAN (2-213): 6.07e-43: 163: 16.94		RNC_HUMAN (1588-1740): 7.08e-17: 89.7: 24.20
+	Hs
		323319 STAU1_HUMAN (13-225): 7.67e-47: 171: 17.72		DICER_HUMAN (1565-1685): 7.57e-12: 73.1: 25.20
+	Hs
		877391 DSRAD_HUMAN (2-213): 6.07e-43: 163: 16.94		DICER_HUMAN (1565-1685): 7.57e-12: 73.1: 25.20
+	Hs
		585588 E2AK2_HUMAN (7-218): 1.31e-43: 162: 19.73		DICER_HUMAN (1565-1685): 7.57e-12: 73.1: 25.20