HitPredict

Interactor 1: PUB3 (O14326)
E3 ubiquitin-protein ligase pub3 (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase pub3)
Gene: PUB3

Interactor 2: UBC4 (P46595)
Ubiquitin-conjugating enzyme E2 4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme 4) (Ubiquitin carrier protein 4) (Ubiquitin-protein ligase 4)
Gene: UBC4

Species: Schizosaccharomyces pombe 972h-

Source: biogrid

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.38	1.00	0.621

Property	Details
Experiments	26771498
Identification Methods	two hybrid
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	HECT~UQ_con
Common GO terms	GO:0000209 - protein polyubiquitination GO:0005737 - cytoplasm GO:0006511 - ubiquitin-dependent protein catabolic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 786 Sp			1 147
+	Sc
		549815 RSP5_YEAST (5-786): 0: 755: 55.70		UBC4_YEAST (3-147): 1.31e-79: 233: 83.45
+	Sc
		549857 RSP5_YEAST (5-786): 0: 755: 55.70		UBC5_YEAST (3-147): 4.83e-79: 231: 82.76
+	Hs
		849452 SMUF1_HUMAN (5-786): 0: 741: 36.02		UB2D1_HUMAN (1-147): 1.88e-82: 240: 79.59
+	Hs
		919869 SMUF1_HUMAN (5-786): 0: 741: 36.02		UB2D2_HUMAN (1-147): 3.47e-82: 239: 84.35
+	Hs
		125808 WWP2_HUMAN (29-786): 0: 736: 36.77		UB2D4_HUMAN (1-147): 2.47e-83: 242: 83.67
+	Hs
		895919 SMUF1_HUMAN (5-786): 0: 741: 36.02		UB2D3_HUMAN (1-147): 3.92e-81: 237: 84.35
+	Hs
		125262 WWP2_HUMAN (29-786): 0: 736: 36.77		UB2D1_HUMAN (1-147): 1.88e-82: 240: 79.59
+	Hs
		125335 WWP2_HUMAN (29-786): 0: 736: 36.77		UB2D2_HUMAN (1-147): 3.47e-82: 239: 84.35
+	Hs
		125300 WWP2_HUMAN (29-786): 0: 736: 36.77		UB2D3_HUMAN (1-147): 3.92e-81: 237: 84.35
+	Hs
		849451 SMUF2_HUMAN (3-786): 0: 730: 34.25		UB2D1_HUMAN (1-147): 1.88e-82: 240: 79.59
+	Hs
		919867 SMUF2_HUMAN (3-786): 0: 730: 34.25		UB2D2_HUMAN (1-147): 3.47e-82: 239: 84.35
+	Hs
		895918 SMUF2_HUMAN (3-786): 0: 730: 34.25		UB2D3_HUMAN (1-147): 3.92e-81: 237: 84.35
+	Hs
		849411 ITCH_HUMAN (136-786): 0: 722: 41.27		UB2D1_HUMAN (1-147): 1.88e-82: 240: 79.59
+	Hs
		919827 ITCH_HUMAN (136-786): 0: 722: 41.27		UB2D2_HUMAN (1-147): 3.47e-82: 239: 84.35
+	Hs
		895882 ITCH_HUMAN (136-786): 0: 722: 41.27		UB2D3_HUMAN (1-147): 3.92e-81: 237: 84.35
+	Hs
		125655 WWP2_HUMAN (29-786): 0: 736: 36.77		UB2E2_HUMAN (4-146): 1.79e-74: 222: 66.43
+	Hs
		125629 WWP2_HUMAN (29-786): 0: 736: 36.77		UB2E3_HUMAN (4-146): 7.18e-74: 221: 66.43
+	Hs
		125263 WWP2_HUMAN (29-786): 0: 736: 36.77		UB2E1_HUMAN (4-146): 1.92e-72: 216: 66.43
+	Hs
		1282329 ITCH_HUMAN (136-786): 0: 722: 41.27		UB2E3_HUMAN (4-146): 7.18e-74: 221: 66.43
+	Hs
		851871 ITCH_HUMAN (136-786): 0: 722: 41.27		UB2E1_HUMAN (4-146): 1.92e-72: 216: 66.43