HitPredict

Interactor 1: RFXK (O14593)
DNA-binding protein RFXANK (Ankyrin repeat family A protein 1) (Regulatory factor X subunit B) (RFX-B) (Regulatory factor X-associated ankyrin-containing protein)
Gene: RFXANK

Interactor 2: RAF1 (P04049)
RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) (Proto-oncogene c-RAF) (cRaf) (Raf-1)
Gene: RAF1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.71	1.00	0.847

Property	Details
Experiments	33961781, 10329666, 37045861
Identification Methods	affinity chromatography technology, enzymatic study, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Ank_2~Pkinase
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0007265 - Ras protein signal transduction GO:0045944 - positive regulation of transcription by RNA polymerase II
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 260 Hs			1 648
+	Hs
		374141 ANK3_HUMAN (29-259): 2.87e-69: 233: 19.47		RAF1_HUMAN (1-648): 0: 652: 100.00
+	Mm
		1307896 RFXK_MOUSE (1-260): 5.16e-76: 233: 81.11		RAF1_MOUSE (1-648): 0: 647: 97.69
+	Rn
		507691 Q5PQM6_RAT (1-260): 5.8e-74: 228: 79.23		RAF1_RAT (1-648): 0: 648: 98.30
+	Hs
		257542 ANR17_HUMAN (4-258): 3.71e-54: 189: 17.24		RAF1_HUMAN (1-648): 0: 652: 100.00
+	Hs
		374070 ANK2_HUMAN (88-245): 6.11e-46: 166: 28.30		RAF1_HUMAN (1-648): 0: 652: 100.00
+	Hs
		373957 RIPK4_HUMAN (61-259): 5.88e-46: 165: 20.00		RAF1_HUMAN (1-648): 0: 652: 100.00
+	Hs
		374864 Q9H4D1_HUMAN (61-259): 9.9e-46: 164: 20.00		RAF1_HUMAN (1-648): 0: 652: 100.00
+	Hs
		374507 ANKH1_HUMAN (2-123): 4.24e-29: 117: 13.82		RAF1_HUMAN (1-648): 0: 652: 100.00
+	Hs
		488633 RIPK4_HUMAN (61-259): 5.88e-46: 165: 20.00		ARAF_HUMAN (45-646): 4.63e-169: 497: 59.28
+	Hs
		488803 Q9H4D1_HUMAN (61-259): 9.9e-46: 164: 20.00		ARAF_HUMAN (45-646): 4.63e-169: 497: 59.28
+	Mm
		93731 E9Q9B7_MOUSE (1-60): 1.87e-09: 60: 14.29		BRAF_MOUSE (38-647): 3.22e-169: 503: 55.52
+	Hs
		883556 RIPK4_HUMAN (61-259): 5.88e-46: 165: 20.00		KSR1_HUMAN (138-648): 6.15e-108: 348: 24.61
+	Hs
		1217986 Q9H4D1_HUMAN (61-259): 9.9e-46: 164: 20.00		KSR1_HUMAN (138-648): 6.15e-108: 348: 24.61
+	Hs
		1217988 TANC2_HUMAN (105-259): 3.1e-22: 97.7: 19.32		KSR1_HUMAN (138-648): 6.15e-108: 348: 24.61