HitPredict

Interactor 1: UB2L6 (O14933)
Ubiquitin/ISG15-conjugating enzyme E2 L6 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme L6) (Retinoic acid-induced gene B protein) (RIG-B) (UbcH8) (Ubiquitin carrier protein L6) (Ubiquitin-protein ligase L6)
Gene: UBE2L6

Interactor 2: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.58	1.00	0.768

Property	Details
Experiments	27684187, 37219487
Identification Methods	affinity chromatography technology, unspecified method, phage display
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	HECT~UQ_con
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0006511 - ubiquitin-dependent protein catabolic process and 1 others more... GO:0045087 - innate immune response
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 153 Hs			1 903
+	Hs
		1294888 UB2D4_HUMAN (2-149): 5.5e-76: 224: 38.93		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Hs
		849411 UB2D1_HUMAN (2-149): 1.04e-75: 223: 38.93		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Hs
		125000 UB2L6_HUMAN (1-153): 2.2e-98: 281: 100.00		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		849444 UB2D1_HUMAN (2-149): 1.04e-75: 223: 38.93		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		159085 UB2L6_HUMAN (1-153): 2.2e-98: 281: 100.00		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		125808 UB2D4_HUMAN (2-149): 5.5e-76: 224: 38.93		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		125262 UB2D1_HUMAN (2-149): 1.04e-75: 223: 38.93		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		125335 UB2D2_HUMAN (2-149): 3.7e-75: 222: 37.58		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		849416 UB2D1_HUMAN (2-149): 1.04e-75: 223: 38.93		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		159031 UB2L6_HUMAN (1-153): 2.2e-98: 281: 100.00		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		808052 UB2D1_HUMAN (2-149): 1.04e-75: 223: 38.93		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		808078 UB2D2_HUMAN (2-149): 3.7e-75: 222: 37.58		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		159070 UB2L6_HUMAN (1-153): 2.2e-98: 281: 100.00		HUWE1_HUMAN (415-902): 1.81e-160: 523: 34.88
+	Hs
		849353 UB2D1_HUMAN (2-149): 1.04e-75: 223: 38.93		HUWE1_HUMAN (415-902): 1.81e-160: 523: 34.88
+	Hs
		919765 UB2D2_HUMAN (2-149): 3.7e-75: 222: 37.58		HUWE1_HUMAN (415-902): 1.81e-160: 523: 34.88
+	Hs
		919782 UB2D2_HUMAN (2-149): 3.7e-75: 222: 37.58		HACE1_HUMAN (376-897): 4.35e-154: 478: 30.61
+	Hs
		849452 UB2D1_HUMAN (2-149): 1.04e-75: 223: 38.93		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		849451 UB2D1_HUMAN (2-149): 1.04e-75: 223: 38.93		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Hs
		849351 UB2D1_HUMAN (2-149): 1.04e-75: 223: 38.93		HECW1_HUMAN (26-479): 2.25e-13: 76.9: 10.76
+	Hs
		919762 UB2D2_HUMAN (2-149): 3.7e-75: 222: 37.58		HECW1_HUMAN (26-479): 2.25e-13: 76.9: 10.76