HitPredict

Interactor 1: ML12B (O14950)
Myosin regulatory light chain 12B (MLC-2A) (MLC-2) (Myosin regulatory light chain 2-B, smooth muscle isoform) (Myosin regulatory light chain 20 kDa) (MLC20) (Myosin regulatory light chain MRLC2) (SHUJUN-1)
Gene: MYL12B

Interactor 2: DAPK3 (O43293)
Death-associated protein kinase 3 (DAP kinase 3) (EC 2.7.11.1) (DAP-like kinase) (Dlk) (MYPT1 kinase) (Zipper-interacting protein kinase) (ZIP-kinase)
Gene: DAPK3

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.41	1.00	0.647

Property	Details
Experiments	21487036
Identification Methods	protein kinase assay
Interaction Type	phosphorylation reaction

Property	Details
Interacting Pfam domains	EF-hand_7~Pkinase
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0008360 - regulation of cell shape
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 172 Hs			1 454
+	Hs
		159558 ML12B_HUMAN (1-172): 1.42e-67: 204: 100.00		DAPK1_HUMAN (1-279): 3.41e-134: 421: 81.36
+	At
		989458 CDPK1_ARATH (62-172): 3.22e-14: 71: 20.18		CDPK1_ARATH (3-444): 5.29e-150: 441: 26.58
+	Gm
		1196117 Q84P28_SOYBN (25-112): 1.15e-10: 61: 18.28		Q84P28_SOYBN (3-444): 2.31e-153: 446: 25.73
+	At
		1105900 CDPKB_ARATH (103-172): 1.13e-12: 66.8: 27.14		CDPKB_ARATH (3-442): 3.88e-149: 435: 24.89
+	At
		1104924 CDPK4_ARATH (37-172): 5.35e-13: 67.6: 19.15		CDPK4_ARATH (3-442): 3.57e-148: 433: 24.66
+	At
		483086 CALM4_ARATH (98-171): 7.33e-17: 74.9: 24.32		CDPK3_ARATH (1-445): 9.92e-144: 422: 28.19
+	At
		483177 CALM4_ARATH (98-171): 7.33e-17: 74.9: 24.32		CDPKU_ARATH (1-445): 2.5e-141: 417: 28.57
+	At
		483166 CALM4_ARATH (98-171): 7.33e-17: 74.9: 24.32		CDPKA_ARATH (6-445): 3.64e-141: 416: 28.44
+	At
		188308 CML8_ARATH (90-171): 3.04e-12: 62.8: 20.73		CDPK3_ARATH (1-445): 9.92e-144: 422: 28.19
+	At
		976625 CALM6_ARATH (32-93): 7.82e-12: 61.7: 31.82		CDPK3_ARATH (1-445): 9.92e-144: 422: 28.19
+	At
		188361 CML8_ARATH (90-171): 3.04e-12: 62.8: 20.73		CDPKU_ARATH (1-445): 2.5e-141: 417: 28.57
+	At
		976699 CALM6_ARATH (32-93): 7.82e-12: 61.7: 31.82		CDPKU_ARATH (1-445): 2.5e-141: 417: 28.57
+	At
		989461 CDPK1_ARATH (62-172): 3.22e-14: 71: 20.18		KIN10_ARATH (7-375): 3.4e-137: 405: 29.73
+	At
		1105921 CDPKB_ARATH (103-172): 1.13e-12: 66.8: 27.14		CDPKO_ARATH (12-429): 2.14e-136: 406: 28.57
+	Hs
		159577 ML12B_HUMAN (1-172): 1.42e-67: 204: 100.00		MYLK_HUMAN (6-308): 2.38e-74: 256: 43.09
+	Hs
		624354 MYL9_HUMAN (1-172): 2.61e-66: 201: 93.02		MYLK_HUMAN (6-308): 2.38e-74: 256: 43.09
+	Gg
		361267 MLRM_CHICK (1-172): 1.14e-65: 199: 92.44		MYLK_CHICK (6-308): 7.25e-75: 257: 43.42
+	Hs
		580651 ML12A_HUMAN (15-172): 2.3e-65: 199: 99.37		MYLK_HUMAN (6-308): 2.38e-74: 256: 43.09
+	Hs
		648500 CALL3_HUMAN (32-93): 8.36e-11: 58.6: 25.76		KCC1A_HUMAN (3-306): 1.58e-132: 388: 37.17
+	Dm
		773033 SQH_DROME (1-172): 2.29e-61: 189: 81.03		Q95SK9_DROME (5-276): 7.78e-69: 227: 41.76