HitPredict

Interactor 1: FYB1 (O15117)
FYN-binding protein 1 (Adhesion and degranulation promoting adaptor protein) (ADAP) (FYB-120/130) (p120/p130) (FYN-T-binding protein) (SLAP-130) (SLP-76-associated phosphoprotein)
Gene: FYB1

Interactor 2: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.68	1.00	0.827

Property	Details
Experiments	25814554, unassigned1126, 22074159
Identification Methods	two hybrid, validated two hybrid, two hybrid array, pull down
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_2, SH3_1~SH3_2, SH3_2~SH3_9
Common GO terms	GO:0005829 - cytosol GO:0044877 - protein-containing complex binding
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 783 Hs			1 380
+	Hs
		167740 FYB1_HUMAN (1-783): 3.31e-92: 308: 100.00		NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24
+	Hs
		167745 FYB1_HUMAN (1-783): 3.31e-92: 308: 100.00		P85A_HUMAN (166-379): 4.05e-24: 106: 16.36
+	Hs
		167737 FYB1_HUMAN (1-783): 3.31e-92: 308: 100.00		FYN_HUMAN (7-137): 2.1e-23: 103: 20.30
+	Hs
		167751 FYB1_HUMAN (1-783): 3.31e-92: 308: 100.00		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Mm
		193090 FYB1_MOUSE (1-783): 7.99e-67: 239: 70.52		FYN_MOUSE (7-137): 2.1e-23: 103: 20.30
+	Hs
		203208 SYNJ1_HUMAN (264-675): 3.42e-09: 62.7: 8.65		NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24
+	Mm
		193088 FYB1_MOUSE (1-783): 7.99e-67: 239: 70.52		LCK_MOUSE (3-188): 2.6e-19: 91.4: 16.58
+	Hs
		411582 MUC1_HUMAN (6-732): 3.39e-28: 124: 9.80		LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53
+	Hs
		554292 MUC1_HUMAN (6-732): 3.39e-28: 124: 9.80		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		522749 MUC1_HUMAN (6-732): 3.39e-28: 124: 9.80		SRC_HUMAN (203-361): 5.72e-16: 81.3: 31.55
+	Hs
		411823 ASPP1_HUMAN (7-477): 1.69e-24: 112: 16.57		LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53
+	Hs
		924760 PRG4_HUMAN (243-720): 1.33e-15: 83.5: 9.28		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		1056926 MDC1_HUMAN (16-730): 7.54e-15: 81.2: 9.50		SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35
+	Hs
		517796 NFH_HUMAN (18-409): 7.32e-12: 71.5: 14.54		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		203228 SYNJ1_HUMAN (264-675): 3.42e-09: 62.7: 8.65		ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23
+	Hs
		203198 SYNJ1_HUMAN (264-675): 3.42e-09: 62.7: 8.65		LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53
+	Hs
		203218 SYNJ1_HUMAN (264-675): 3.42e-09: 62.7: 8.65		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		203230 SYNJ1_HUMAN (264-675): 3.42e-09: 62.7: 8.65		SH319_HUMAN (1-57): 2.18e-16: 82.8: 19.30
+	Hs
		203241 SYNJ1_HUMAN (264-675): 3.42e-09: 62.7: 8.65		SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35