HitPredict

Interactor 1: TRI18 (O15344)
E3 ubiquitin-protein ligase Midline-1 (EC 2.3.2.27) (Midin) (Putative transcription factor XPRF) (RING finger protein 59) (RING finger protein Midline-1) (RING-type E3 ubiquitin transferase Midline-1) (Tripartite motif-containing protein 18)
Gene: MID1

Interactor 2: IL6RA (P08887)
Interleukin-6 receptor subunit alpha (IL-6 receptor subunit alpha) (IL-6R subunit alpha) (IL-6R-alpha) (IL-6RA) (IL-6R 1) (Membrane glycoprotein 80) (gp80) (CD antigen CD126) [Cleaved into: Soluble interleukin-6 receptor subunit alpha (sIL6R)]
Gene: IL6R

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.65	1.00	0.811

Property	Details
Experiments	28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	fn3~ig, IL6Ra-bind~fn3
Common GO terms	GO:0019899 - enzyme binding GO:0042803 - protein homodimerization activity
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 667 Hs			1 468
+	Hs
		1201372 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		IGFN1_HUMAN (179-411): 2.09e-12: 71.5: 11.74
+	Hs
		645943 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		CNTFR_HUMAN (31-368): 2.92e-62: 208: 25.51
+	Hs
		770203 CMYA5_HUMAN (160-661): 1.55e-72: 258: 19.63		IL6RB_HUMAN (1-364): 1.51e-73: 250: 17.19
+	Hs
		685317 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		L1CAM_HUMAN (58-403): 5.7e-23: 104: 11.38
+	Hs
		489979 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		PTPRF_HUMAN (47-290): 1.62e-12: 72.3: 12.55
+	Hs
		1018298 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		CNTN1_HUMAN (13-155): 1.74e-12: 71.9: 15.75
+	Hs
		1171073 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		NRCAM_HUMAN (18-429): 5.54e-10: 63.8: 12.01
+	Hs
		1171020 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		TITIN_HUMAN (17-203): 4.45e-09: 61.5: 13.27
+	Hs
		1171077 TRI67_HUMAN (54-660): 7.29e-91: 301: 24.39		NEO1_HUMAN (28-133): 4.31e-09: 61.1: 19.84
+	Hs
		1264780 TRI63_HUMAN (1-380): 2.91e-86: 276: 24.09		TITIN_HUMAN (17-203): 4.45e-09: 61.5: 13.27
+	Hs
		1201357 TRI42_HUMAN (4-402): 2.29e-77: 263: 22.14		IGFN1_HUMAN (179-411): 2.09e-12: 71.5: 11.74
+	Hs
		1264797 TRI55_HUMAN (1-378): 3.73e-80: 266: 23.44		TITIN_HUMAN (17-203): 4.45e-09: 61.5: 13.27
+	Hs
		1264796 TRI54_HUMAN (1-380): 7.45e-80: 259: 22.80		TITIN_HUMAN (17-203): 4.45e-09: 61.5: 13.27
+	Hs
		536902 TRI27_HUMAN (90-433): 1.46e-30: 128: 14.08		IGFN1_HUMAN (179-411): 2.09e-12: 71.5: 11.74
+	Hs
		585001 RO52_HUMAN (1-295): 2.16e-18: 90.5: 12.70		IGFN1_HUMAN (179-411): 2.09e-12: 71.5: 11.74
+	Hs
		734565 TRI23_HUMAN (8-252): 7.45e-19: 92.4: 25.88		MYOM1_HUMAN (10-282): 6.31e-11: 66.9: 10.77