HitPredict

Interactor 1: TRI18 (O15344)
E3 ubiquitin-protein ligase Midline-1 (EC 2.3.2.27) (Midin) (Putative transcription factor XPRF) (RING finger protein 59) (RING finger protein Midline-1) (RING-type E3 ubiquitin transferase Midline-1) (Tripartite motif-containing protein 18)
Gene: MID1

Interactor 2: UBE2W (Q96B02)
Ubiquitin-conjugating enzyme E2 W (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme W) (N-terminal E2 ubiquitin-conjugating enzyme) (EC 2.3.2.25) (N-terminus-conjugating E2) (Ubiquitin carrier protein W) (Ubiquitin-conjugating enzyme 16) (UBC-16) (Ubiquitin-protein ligase W)
Gene: UBE2W

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	1.00	0.698

Property	Details
Experiments	19549727
Identification Methods	two hybrid, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	GO:0005737 - cytoplasm GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 667 Hs			1 151
+	Hs
		175730 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37
+	Hs
		175653 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69
+	Hs
		175664 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2D2_HUMAN (4-150): 1.14e-68: 205: 33.33
+	Hs
		175658 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2D3_HUMAN (4-150): 3.12e-67: 202: 33.33
+	Hs
		175710 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2E2_HUMAN (1-150): 3.17e-66: 201: 34.67
+	Hs
		175703 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2E3_HUMAN (1-150): 1.13e-65: 200: 35.33
+	Hs
		175654 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2E1_HUMAN (1-150): 3.64e-64: 196: 34.00
+	Hs
		1151762 Q6GX22_HUMAN (1-665): 1.26e-170: 505: 76.24		UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37
+	Hs
		1356055 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37
+	Hs
		849480 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69
+	Hs
		919896 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D2_HUMAN (4-150): 1.14e-68: 205: 33.33
+	Hs
		895945 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D3_HUMAN (4-150): 3.12e-67: 202: 33.33
+	Hs
		1297378 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2E2_HUMAN (1-150): 3.17e-66: 201: 34.67
+	Hs
		1282363 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2E3_HUMAN (1-150): 1.13e-65: 200: 35.33
+	Hs
		851905 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2E1_HUMAN (1-150): 3.64e-64: 196: 34.00
+	Hs
		1052807 TRI25_HUMAN (4-656): 9.25e-108: 341: 22.80		UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37
+	Hs
		849330 TRI25_HUMAN (4-656): 9.25e-108: 341: 22.80		UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69
+	Hs
		919727 TRI25_HUMAN (4-656): 9.25e-108: 341: 22.80		UB2D2_HUMAN (4-150): 1.14e-68: 205: 33.33
+	Hs
		895794 TRI25_HUMAN (4-656): 9.25e-108: 341: 22.80		UB2D3_HUMAN (4-150): 3.12e-67: 202: 33.33
+	Hs
		919872 TRI36_HUMAN (1-647): 2.78e-95: 311: 24.59		UB2D2_HUMAN (4-150): 1.14e-68: 205: 33.33