HitPredict

Interactor 1: PDPK1 (O15530)
3-phosphoinositide-dependent protein kinase 1 (hPDK1) (EC 2.7.11.1)
Gene: PDPK1

Interactor 2: 1433G (P61981)
14-3-3 protein gamma (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein gamma, N-terminally processed]
Gene: YWHAG

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.66	0.60	0.633

Property	Details
Experiments	31980649, 36736316, unassigned2173
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005925 - focal adhesion GO:0006469 - negative regulation of protein kinase activity GO:0032869 - cellular response to insulin stimulus
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 556 Hs			1 247
+	Hs
		182125 PDPK1_HUMAN (1-556): 0: 563: 100.00		1433E_HUMAN (1-244): 2.02e-141: 398: 63.53
+	Hs
		182139 PDPK1_HUMAN (1-556): 0: 563: 100.00		1433F_HUMAN (1-246): 2.5e-135: 382: 86.99
+	Hs
		182098 PDPK1_HUMAN (1-556): 0: 563: 100.00		1433B_HUMAN (2-247): 1.91e-109: 317: 75.40
+	Hs
		182127 PDPK1_HUMAN (1-556): 0: 563: 100.00		1433Z_HUMAN (2-245): 2.39e-108: 314: 74.59
+	Hs
		182088 PDPK1_HUMAN (1-556): 0: 563: 100.00		1433T_HUMAN (2-245): 5.53e-105: 305: 70.33
+	Hs
		182099 PDPK1_HUMAN (1-556): 0: 563: 100.00		1433S_HUMAN (2-245): 1.86e-98: 289: 65.16
+	At
		965291 CDPK1_ARATH (2-533): 5.4e-146: 435: 19.82		14332_ARATH (4-244): 1.39e-141: 398: 61.32
+	At
		802001 CDPK1_ARATH (2-533): 5.4e-146: 435: 19.82		14334_ARATH (4-244): 5e-140: 395: 60.91
+	At
		788694 CDPK1_ARATH (2-533): 5.4e-146: 435: 19.82		14335_ARATH (3-244): 5.67e-136: 385: 59.02
+	Hs
		681770 AKT1_HUMAN (46-410): 1.08e-141: 419: 31.35		1433E_HUMAN (1-244): 2.02e-141: 398: 63.53
+	At
		1280891 CIPKC_ARATH (59-495): 8.42e-140: 415: 22.89		14337_ARATH (3-244): 3.18e-142: 400: 60.25
+	At
		788672 CIPKC_ARATH (59-495): 8.42e-140: 415: 22.89		14333_ARATH (1-244): 4.4e-142: 400: 61.79
+	At
		965360 CIPKC_ARATH (59-495): 8.42e-140: 415: 22.89		14332_ARATH (4-244): 1.39e-141: 398: 61.32
+	Hs
		681766 AKT1_HUMAN (46-410): 1.08e-141: 419: 31.35		1433G_HUMAN (1-247): 3.15e-139: 392: 100.00
+	Hs
		568616 KAPCA_HUMAN (40-391): 7.05e-139: 407: 31.53		1433E_HUMAN (1-244): 2.02e-141: 398: 63.53
+	Hs
		681776 AKT1_HUMAN (46-410): 1.08e-141: 419: 31.35		1433Z_HUMAN (2-245): 2.39e-108: 314: 74.59
+	Hs
		568620 KAPCA_HUMAN (40-391): 7.05e-139: 407: 31.53		1433Z_HUMAN (2-245): 2.39e-108: 314: 74.59
+	Mm
		926770 KAPCB_MOUSE (72-391): 4.52e-137: 403: 34.69		1433Z_MOUSE (2-245): 8.46e-109: 315: 74.59
+	Hs
		610682 KAPCB_HUMAN (72-391): 6.83e-138: 405: 34.38		1433T_HUMAN (2-245): 5.53e-105: 305: 70.33
+	Hs
		681682 AKT1_HUMAN (46-410): 1.08e-141: 419: 31.35		1433S_HUMAN (2-245): 1.86e-98: 289: 65.16