HitPredict

Interactor 1: ALX4 (O35137)
Homeobox protein aristaless-like 4 (ALX-4)
Gene: ALX4

Interactor 2: MIXL1 (Q9WUI0)
Homeobox protein MIXL1 (Homeodomain protein MIX) (mMix) (MIX1 homeobox-like protein 1) (Mix.1 homeobox-like protein)
Gene: MIXL1

Species: Mus musculus

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	1.00	0.698

Property	Details
Experiments	20211142
Identification Methods	two hybrid, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Homeodomain~Homeodomain
Common GO terms	GO:0001228 - DNA-binding transcription activator activity, RNA polymerase II-specific GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0006357 - regulation of transcription by RNA polymerase II GO:0045944 - positive regulation of transcription by RNA polymerase II and 1 others more... GO:0048565 - digestive tract development
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 399 Mm			1 231
+	Mm
		192275 ALX4_MOUSE (1-399): 2.11e-84: 264: 100.00		ALX4_MOUSE (2-222): 2.76e-36: 133: 21.27
+	Mm
		192304 ALX4_MOUSE (1-399): 2.11e-84: 264: 100.00		Q8VD35_MOUSE (54-142): 8.22e-35: 127: 47.19
+	Hs
		1328470 ALX4_HUMAN (1-399): 2.17e-79: 252: 89.78		ALX4_HUMAN (2-222): 5.85e-36: 132: 21.27
+	Hs
		1077278 ALX4_HUMAN (1-399): 2.17e-79: 252: 89.78		ALX1_HUMAN (51-230): 2.38e-36: 131: 29.44
+	Mm
		192297 ALX4_MOUSE (1-399): 2.11e-84: 264: 100.00		HXC4_MOUSE (13-181): 5.04e-32: 119: 19.57
+	Mm
		192276 ALX4_MOUSE (1-399): 2.11e-84: 264: 100.00		RX_MOUSE (22-227): 5.32e-31: 118: 24.42
+	Mm
		192281 ALX4_MOUSE (1-399): 2.11e-84: 264: 100.00		PAX3_MOUSE (44-230): 5.85e-30: 117: 26.04
+	Mm
		192277 ALX4_MOUSE (1-399): 2.11e-84: 264: 100.00		LMX1B_MOUSE (45-218): 4.95e-30: 116: 18.79
+	Mm
		192303 ALX4_MOUSE (1-399): 2.11e-84: 264: 100.00		Q8R0B5_MOUSE (66-142): 2.82e-13: 69.3: 49.41
+	Mm
		837781 NKX21_MOUSE (24-373): 3.13e-38: 143: 19.33		MIXL1_MOUSE (1-231): 2.4e-48: 160: 100.00
+	Hs
		678782 HXD3_HUMAN (8-376): 4.7e-39: 146: 17.82		ALX4_HUMAN (2-222): 5.85e-36: 132: 21.27
+	Mm
		837779 NKX21_MOUSE (24-373): 3.13e-38: 143: 19.33		VSX1_MOUSE (39-147): 7.23e-37: 134: 37.93
+	Hs
		656400 PIT1_HUMAN (2-267): 2.75e-39: 143: 13.03		PITX1_HUMAN (33-148): 2.9e-36: 131: 37.01
+	Dm
		949988 AL_DROME (159-383): 2.47e-39: 146: 29.03		UBX_DROME (29-150): 6.26e-30: 116: 22.13
+	Hs
		174008 SHOX_HUMAN (191-397): 2.79e-37: 138: 28.50		SHOX_HUMAN (75-148): 2.51e-33: 123: 45.95
+	Dm
		185927 PITX_DROME (178-394): 1.87e-32: 129: 29.22		UBX_DROME (29-150): 6.26e-30: 116: 22.13
+	Hs
		941950 PITX1_HUMAN (138-393): 1.88e-33: 128: 28.04		PITX2_HUMAN (43-216): 1.05e-29: 114: 25.57