HitPredict

Interactor 1: ASAP2 (O43150)
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 (Development and differentiation-enhancing factor 2) (Paxillin-associated protein with ARF GAP activity 3) (PAG3) (Pyk2 C-terminus-associated protein) (PAP)
Gene: ASAP2

Interactor 2: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.789

Property	Details
Experiments	33961781, 17474147
Identification Methods	affinity chromatography technology, peptide array, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Ank_2~SH3_1, PH~SH3_1
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1006 Hs			1 377
+	Hs
		195770 ASAP2_HUMAN (1-1006): 0: 661: 100.00		NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24
+	Hs
		195787 ASAP2_HUMAN (1-1006): 0: 661: 100.00		P85A_HUMAN (241-376): 1.37e-22: 101: 19.57
+	Hs
		195776 ASAP2_HUMAN (1-1006): 0: 661: 100.00		FYN_HUMAN (7-173): 7.47e-21: 96: 15.39
+	Hs
		195795 ASAP2_HUMAN (1-1006): 0: 661: 100.00		ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07
+	Hs
		195782 ASAP2_HUMAN (1-1006): 0: 661: 100.00		SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32
+	Hs
		195791 ASAP2_HUMAN (1-1006): 0: 661: 100.00		GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81
+	Hs
		195777 ASAP2_HUMAN (1-1006): 0: 661: 100.00		YES_HUMAN (112-218): 4.18e-11: 66.1: 19.27
+	Hs
		195796 ASAP2_HUMAN (1-1006): 0: 661: 100.00		Q5JPT6_HUMAN (58-207): 2.14e-09: 61.3: 17.76
+	Hs
		195804 ASAP2_HUMAN (1-1006): 0: 661: 100.00		SH3K1_HUMAN (58-207): 2.14e-09: 61.3: 17.76
+	Hs
		557696 ACAP1_HUMAN (23-678): 3.93e-128: 407: 19.97		NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00
+	Hs
		522992 ACAP1_HUMAN (23-678): 3.93e-128: 407: 19.97		SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32
+	Hs
		924582 ACAP1_HUMAN (23-678): 3.93e-128: 407: 19.97		GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81
+	Hs
		523208 AGAP1_HUMAN (136-646): 6.55e-94: 319: 17.69		SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32
+	Mm
		1112566 AGAP2_MOUSE (136-646): 5.93e-79: 282: 18.39		SRBS2_MOUSE (46-288): 1.38e-24: 107: 18.85
+	Hs
		655450 AGAP2_HUMAN (136-646): 1.27e-80: 287: 18.58		P85A_HUMAN (241-376): 1.37e-22: 101: 19.57
+	Mm
		70554 AGAP2_MOUSE (136-646): 5.93e-79: 282: 18.39		B2RQ57_MOUSE (197-356): 1.08e-15: 80.9: 33.95
+	Mm
		403434 AGAP2_MOUSE (136-646): 5.93e-79: 282: 18.39		SRC_MOUSE (5-114): 1.55e-15: 79.7: 22.32
+	Hs
		655498 DP13A_HUMAN (24-438): 1.35e-70: 251: 16.51		P85A_HUMAN (241-376): 1.37e-22: 101: 19.57
+	Hs
		129347 DP13A_HUMAN (24-438): 1.35e-70: 251: 16.51		P85B_HUMAN (100-376): 1.85e-21: 98.3: 16.61
+	Hs
		412284 ARAP2_HUMAN (120-625): 1.74e-59: 225: 19.77		FYN_HUMAN (7-173): 7.47e-21: 96: 15.39