HitPredict

Interactor 1: PJA2 (O43164)
E3 ubiquitin-protein ligase Praja-2 (Praja2) (EC 2.3.2.27) (RING finger protein 131) (RING-type E3 ubiquitin transferase Praja-2)
Gene: PJA2

Interactor 2: UBE2W (Q96B02)
Ubiquitin-conjugating enzyme E2 W (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme W) (N-terminal E2 ubiquitin-conjugating enzyme) (EC 2.3.2.25) (N-terminus-conjugating E2) (Ubiquitin carrier protein W) (Ubiquitin-conjugating enzyme 16) (UBC-16) (Ubiquitin-protein ligase W)
Gene: UBE2W

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	1.00	0.698

Property	Details
Experiments	19690564
Identification Methods	two hybrid, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_2
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005737 - cytoplasm GO:0043161 - proteasome-mediated ubiquitin-dependent protein catabolic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 708 Hs			1 151
+	Hs
		196372 PJA2_HUMAN (1-708): 0: 580: 100.00		UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37
+	Hs
		196282 PJA2_HUMAN (1-708): 0: 580: 100.00		UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69
+	Hs
		196294 PJA2_HUMAN (1-708): 0: 580: 100.00		UB2D2_HUMAN (4-150): 1.14e-68: 205: 33.33
+	Hs
		196289 PJA2_HUMAN (1-708): 0: 580: 100.00		UB2D3_HUMAN (4-150): 3.12e-67: 202: 33.33
+	Hs
		919792 PJA1_HUMAN (12-681): 2.38e-128: 396: 42.92		UB2D2_HUMAN (4-150): 1.14e-68: 205: 33.33
+	Hs
		895852 PJA1_HUMAN (12-681): 2.38e-128: 396: 42.92		UB2D3_HUMAN (4-150): 3.12e-67: 202: 33.33
+	Hs
		1245284 PJA1_HUMAN (12-681): 2.38e-128: 396: 42.92		UB2E3_HUMAN (1-150): 1.13e-65: 200: 35.33
+	At
		96605 F4IQ07_ARATH (145-687): 4.23e-61: 217: 14.34		UBC9_ARATH (4-150): 2.28e-68: 205: 32.65
+	At
		96610 F4IQ07_ARATH (145-687): 4.23e-61: 217: 14.34		UBC29_ARATH (4-150): 2.73e-66: 200: 30.61
+	At
		715138 Q8VYL4_ARATH (145-687): 1.69e-56: 203: 14.34		UBC9_ARATH (4-150): 2.28e-68: 205: 32.65
+	At
		1258030 Q8VYL4_ARATH (145-687): 1.69e-56: 203: 14.34		UBC29_ARATH (4-150): 2.73e-66: 200: 30.61
+	At
		96607 F4IQ07_ARATH (145-687): 4.23e-61: 217: 14.34		UBC18_ARATH (4-149): 1.76e-59: 183: 50.00
+	At
		96608 F4IQ07_ARATH (145-687): 4.23e-61: 217: 14.34		UBC16_ARATH (7-149): 1.8e-59: 183: 51.72
+	Hs
		849459 RNF12_HUMAN (124-679): 2.25e-50: 188: 15.36		UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69
+	Hs
		919877 RNF12_HUMAN (124-679): 2.25e-50: 188: 15.36		UB2D2_HUMAN (4-150): 1.14e-68: 205: 33.33
+	Hs
		895927 RNF12_HUMAN (124-679): 2.25e-50: 188: 15.36		UB2D3_HUMAN (4-150): 3.12e-67: 202: 33.33
+	Hs
		1297371 RNF12_HUMAN (124-679): 2.25e-50: 188: 15.36		UB2E2_HUMAN (1-150): 3.17e-66: 201: 34.67
+	At
		1258027 Q8VYL4_ARATH (145-687): 1.69e-56: 203: 14.34		UBC18_ARATH (4-149): 1.76e-59: 183: 50.00
+	At
		1258028 Q8VYL4_ARATH (145-687): 1.69e-56: 203: 14.34		UBC16_ARATH (7-149): 1.8e-59: 183: 51.72
+	Hs
		851893 RNF12_HUMAN (124-679): 2.25e-50: 188: 15.36		UB2E1_HUMAN (1-150): 3.64e-64: 196: 34.00