HitPredict

Interactor 1: RGS14 (O43566)
Regulator of G-protein signaling 14 (RGS14)
Gene: RGS14

Interactor 2: GNAI1 (P63096)
Guanine nucleotide-binding protein G(i) subunit alpha-1 (Adenylate cyclase-inhibiting G alpha protein)
Gene: GNAI1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	1.00	0.966

Property	Details
Experiments	11976690, 11387333, 26186194, 28514442, 33961781, 19899154, 21988832
Identification Methods	x-ray crystallography, two hybrid, affinity chromatography technology, pull down, two hybrid array, anti tag coimmunoprecipitation
Interaction Type	association, direct interaction, physical association

Property	Details
Interacting Pfam domains	G-alpha~GoLoco, G-alpha~RGS
Common GO terms	GO:0003924 - GTPase activity GO:0005737 - cytoplasm GO:0005813 - centrosome GO:0005886 - plasma membrane GO:0007186 - G protein-coupled receptor signaling pathway and 1 others more... GO:0051301 - cell division
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 566 Hs			1 354
+	Hs
		207507 RGS14_HUMAN (1-566): 0: 656: 100.00		GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79
+	Hs
		207505 RGS14_HUMAN (1-566): 0: 656: 100.00		GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89
+	Hs
		207511 RGS14_HUMAN (1-566): 0: 656: 100.00		GNA11_HUMAN (1-353): 1.33e-162: 460: 51.13
+	Mm
		579646 RGS14_MOUSE (1-545): 0: 594: 87.93		GNAO_MOUSE (1-354): 1.59e-172: 485: 71.83
+	Hs
		158608 RGS12_HUMAN (3-562): 7.98e-167: 513: 36.61		GNAI1_HUMAN (1-354): 0: 512: 100.00
+	Hs
		158599 RGS12_HUMAN (3-562): 7.98e-167: 513: 36.61		GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79
+	Hs
		158597 RGS12_HUMAN (3-562): 7.98e-167: 513: 36.61		GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89
+	Dm
		592744 RGS_DROME (7-514): 7.23e-131: 418: 28.41		GNAI_DROME (1-354): 1.9e-168: 475: 77.75
+	Dm
		558483 RGS_DROME (7-514): 7.23e-131: 418: 28.41		GNAO_DROME (1-354): 6.39e-166: 468: 69.01
+	Hs
		170629 AXIN1_HUMAN (1-494): 2.48e-147: 447: 18.60		GNAS2_HUMAN (1-354): 3.5e-143: 412: 38.23
+	Hs
		444693 RGS7_HUMAN (6-199): 9.43e-65: 221: 27.04		GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79
+	Hs
		926720 RGS18_HUMAN (51-216): 1.23e-61: 205: 31.74		GNAI1_HUMAN (1-354): 0: 512: 100.00
+	Hs
		277782 RGS9_HUMAN (28-212): 1.28e-62: 219: 30.65		GNAO_HUMAN (1-354): 4.45e-175: 491: 72.11
+	Hs
		444800 RGS18_HUMAN (51-216): 1.23e-61: 205: 31.74		GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79
+	Hs
		391473 RGS18_HUMAN (51-216): 1.23e-61: 205: 31.74		GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89
+	Hs
		180650 RGS16_HUMAN (8-183): 4.71e-62: 204: 33.33		GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89
+	Bt
		221230 RGS9_BOVIN (28-212): 4.67e-66: 224: 31.18		GNAT1_BOVIN (1-354): 8.16e-169: 475: 67.80
+	Hs
		834946 RGS7_HUMAN (6-199): 9.43e-65: 221: 27.04		GNAQ_HUMAN (1-353): 3.49e-167: 472: 52.26
+	Hs
		837653 RGS18_HUMAN (51-216): 1.23e-61: 205: 31.74		GNAQ_HUMAN (1-353): 3.49e-167: 472: 52.26
+	Hs
		580410 RGS7_HUMAN (6-199): 9.43e-65: 221: 27.04		GNAZ_HUMAN (1-353): 2.73e-160: 454: 67.23