HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: AFAD (P55196)
Afadin (ALL1-fused gene from chromosome 6 protein) (Protein AF-6) (Afadin adherens junction formation factor)
Gene: AFDN

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	0.60	0.667

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0007165 - signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 1824
+	Hs
		876349 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		AFAD_HUMAN (1-1824): 0: 1279: 100.00
+	Hs
		557789 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		KI13A_HUMAN (184-691): 2.35e-70: 264: 13.74
+	Mm
		223014 NCK2_MOUSE (1-380): 3.58e-98: 298: 96.05		DLG4_MOUSE (1002-1238): 8.46e-28: 123: 17.51
+	Hs
		655462 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		KI13A_HUMAN (184-691): 2.35e-70: 264: 13.74
+	Hs
		454833 FGR_HUMAN (7-155): 1.02e-23: 104: 18.54		KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67
+	Hs
		412200 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67
+	Hs
		442832 HCK_HUMAN (2-145): 5.1e-23: 102: 24.83		KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67
+	Hs
		411726 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67
+	Hs
		442887 HCK_HUMAN (2-145): 5.1e-23: 102: 24.83		GOPC_HUMAN (744-1099): 5.55e-40: 157: 14.72
+	Hs
		412128 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		DLG4_HUMAN (1002-1238): 1.07e-27: 123: 17.51
+	Mm
		403445 SRC_MOUSE (6-153): 5.44e-20: 93.3: 17.33		DLG4_MOUSE (1002-1238): 8.46e-28: 123: 17.51
+	Mm
		1141133 GRB2_MOUSE (1-61): 1.04e-18: 85.9: 27.42		DLG4_MOUSE (1002-1238): 8.46e-28: 123: 17.51
+	Hs
		924466 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		DLG4_HUMAN (1002-1238): 1.07e-27: 123: 17.51
+	Hs
		924792 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		RADIL_HUMAN (830-1106): 4.06e-27: 122: 18.06
+	Hs
		655420 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		LNX2_HUMAN (908-1089): 8.99e-18: 91.8: 18.13
+	Hs
		411718 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		SCRIB_HUMAN (989-1133): 4.27e-22: 106: 26.85
+	Hs
		411696 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		PTN13_HUMAN (978-1122): 1.17e-17: 92.2: 29.14
+	Hs
		924701 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		LNX2_HUMAN (908-1089): 8.99e-18: 91.8: 18.13
+	Hs
		411905 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		MYO5A_HUMAN (1369-1800): 2.36e-11: 71.5: 10.74
+	Hs
		924586 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		PLEC_HUMAN (1384-1680): 9.8e-11: 69.6: 16.67