HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: BCAR1 (P56945)
Breast cancer anti-estrogen resistance protein 1 (CRK-associated substrate) (Cas scaffolding protein family member 1) (p130cas)
Gene: BCAR1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.61	1.00	0.783

Property	Details
Experiments	32296183, unassigned1304
Identification Methods	two hybrid, two hybrid array, validated two hybrid, two hybrid prey pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007015 - actin filament organization GO:0007173 - epidermal growth factor receptor signaling pathway GO:0016477 - cell migration
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 870
+	Hs
		557645 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		BCAR1_HUMAN (1-870): 2.49e-107: 352: 100.00
+	Hs
		557691 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		CASL_HUMAN (1-868): 1.02e-91: 309: 35.96
+	Hs
		655289 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		BCAR1_HUMAN (1-870): 2.49e-107: 352: 100.00
+	Rn
		1144371 P85A_RAT (163-379): 8.5e-24: 105: 16.14		BCAR1_RAT (2-870): 4.08e-103: 343: 89.12
+	Hs
		209039 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ACK1_HUMAN (9-499): 2.55e-30: 131: 15.35
+	Hs
		557670 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ACK1_HUMAN (9-499): 2.55e-30: 131: 15.35
+	Mm
		222878 NCK1_MOUSE (1-379): 6.79e-91: 279: 68.24		ACK1_MOUSE (9-581): 1.34e-29: 128: 13.93
+	Hs
		199489 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		EFS_HUMAN (4-429): 4.35e-14: 78: 22.56
+	Hs
		974160 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		DYST_HUMAN (457-866): 1.71e-67: 248: 12.07
+	Hs
		199501 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		EFS_HUMAN (4-429): 4.35e-14: 78: 22.56
+	Hs
		203208 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		SYNJ1_HUMAN (281-696): 4.9e-10: 65.7: 13.47
+	Hs
		3132 A0A0S2Z4D7_HUMAN (1-138): 6.51e-59: 190: 67.39		EFS_HUMAN (4-429): 4.35e-14: 78: 22.56
+	Hs
		782781 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		ACK1_HUMAN (9-499): 2.55e-30: 131: 15.35
+	Hs
		655334 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		ACK1_HUMAN (9-499): 2.55e-30: 131: 15.35
+	Hs
		91474 E7ERP6_HUMAN (1-76): 8.84e-45: 150: 98.68		EFS_HUMAN (4-429): 4.35e-14: 78: 22.56
+	Hs
		298006 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		FCSD2_HUMAN (3-156): 3.32e-24: 110: 24.68
+	Hs
		1285205 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		SH3K1_HUMAN (7-294): 1.08e-16: 86.7: 16.44
+	Hs
		655439 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		SH3K1_HUMAN (7-294): 1.08e-16: 86.7: 16.44
+	Hs
		199505 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		EFS_HUMAN (4-429): 4.35e-14: 78: 22.56
+	Hs
		257713 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		ANR17_HUMAN (67-718): 5.11e-09: 62.6: 9.52