HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: GAB1 (Q13480)
GRB2-associated-binding protein 1 (GRB2-associated binder 1) (Growth factor receptor bound protein 2-associated protein 1)
Gene: GAB1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.692

Property	Details
Experiments	24728074
Identification Methods	pull down, fluorescence polarization spectroscopy
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	PH~SH3_1, PH~SH3_9
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007165 - signal transduction GO:0007173 - epidermal growth factor receptor signaling pathway GO:0035591 - signaling adaptor activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 694
+	Hs
		557681 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Hs
		557834 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		GAB2_HUMAN (3-691): 2.24e-153: 461: 39.01
+	Hs
		655356 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Mm
		643113 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		GAB1_MOUSE (1-694): 0: 544: 89.93
+	Hs
		129290 P85B_HUMAN (21-380): 7.78e-23: 102: 13.10		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Hs
		442825 HCK_HUMAN (2-145): 5.1e-23: 102: 24.83		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Hs
		846468 BLK_HUMAN (7-204): 3.35e-19: 91: 19.80		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Mm
		403466 SRC_MOUSE (6-153): 5.44e-20: 93.3: 17.33		GAB1_MOUSE (1-694): 0: 544: 89.93
+	Hs
		411709 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Hs
		1040322 GRAP_HUMAN (5-169): 7.3e-28: 110: 15.74		GAB2_HUMAN (3-691): 2.24e-153: 461: 39.01
+	Hs
		655510 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		GAB2_HUMAN (3-691): 2.24e-153: 461: 39.01
+	Hs
		129351 P85B_HUMAN (21-380): 7.78e-23: 102: 13.10		GAB2_HUMAN (3-691): 2.24e-153: 461: 39.01
+	Mm
		69824 B1AT92_MOUSE (5-171): 1.06e-28: 112: 19.80		GAB2_MOUSE (3-691): 8.7e-141: 429: 38.33
+	Mm
		643089 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		Q3ZB59_MOUSE (3-691): 1.2e-140: 428: 38.33
+	Mm
		140067 P85B_MOUSE (21-380): 4.92e-23: 102: 13.10		GAB2_MOUSE (3-691): 8.7e-141: 429: 38.33
+	Mm
		140061 P85B_MOUSE (21-380): 4.92e-23: 102: 13.10		Q3ZB59_MOUSE (3-691): 1.2e-140: 428: 38.33
+	Hs
		412280 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		GAB3_HUMAN (1-683): 1.81e-103: 329: 28.82
+	Hs
		411764 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		PKHA7_HUMAN (5-485): 5.86e-74: 260: 12.07
+	Hs
		411861 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		PKHA5_HUMAN (5-476): 6.15e-67: 240: 13.00
+	Hs
		411897 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		PKHA6_HUMAN (5-495): 5.54e-58: 214: 13.33